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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/129134
Título

The nucleolar protein Viriato/Nol12 is required for the growth and differentiation progression activities of the Dpp pathway during Drosophila eye development

AutorMarinho, Joana; Martins, Torcato; Neto, Marta; Casares, Fernando ; Pereira, Paulo S.
Palabras claveDrosophila
Cell proliferation
Photoreceptor differentiation
Dpp/TGFbeta
Viriato/Nol12
Fecha de publicación2013
EditorElsevier
CitaciónDevelopmental Biology 377(1): 154-165 (2013)
ResumenDrosophila Decapentaplegic (Dpp), a member of the BMP2/4 class of the TGF-βs, is required for organ growth, patterning and differentiation. However, much remains to be understood about the mechanisms acting downstream of these multiple roles. Here we investigate this issue during the development of the Drosophila eye. We have previously identified viriato (vito) as a dMyc-target gene encoding a nucleolar protein that is required for proper tissue growth in the developing eye. By carrying out a targeted in vivo double-RNAi screen to identify genes and pathways functioning with Vito during eye development, we found a strong genetic interaction between vito and members of the Dpp signaling pathway including the TGF-β receptors tkv (type I), put (type II), and the co-Smad medea (med). Analyzing the expression of the Dpp receptor Tkv and the activation pattern of the pathway’s transducer, p-Mad, we found that vito is required for a correct signal transduction in Dpp-receiving cells. Overall, we validate the use of double RNAi to find specific genetic interactions and, in particular, we uncover a link between the Dpp pathway and Vito, a nucleolar component. vito would act genetically downstream of Dpp, playing an important role in maintaining a sufficient level of Dpp activity for the promotion of eye disc growth and regulation of photoreceptor differentiation in eye development.
Versión del editorhttp://dx.doi.org/10.1016/j.ydbio.2013.02.003
URIhttp://hdl.handle.net/10261/129134
DOI10.1016/j.ydbio.2013.02.003
ISSN0012-1606
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