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dc.contributor.authorBogdanovic, Ozrenes_ES
dc.contributor.authorFernández-Miñán, Anaes_ES
dc.contributor.authorTena, Juan J.es_ES
dc.contributor.authorCalle-Mustienes, Elisa de laes_ES
dc.contributor.authorGómez-Skarmeta, José Luises_ES
dc.date.accessioned2016-02-16T12:02:50Z-
dc.date.available2016-02-16T12:02:50Z-
dc.date.issued2013-
dc.identifier.citationMethods 62(3): 207-215 (2013)es_ES
dc.identifier.issn1046-2023-
dc.identifier.urihttp://hdl.handle.net/10261/129126-
dc.description.abstractGenome-wide profiling of DNA methylation and histone modifications answered many questions as to how the genes are regulated on a global scale and what their epigenetic makeup is. Yet, little is known about the function of these marks during early vertebrate embryogenesis. Here we provide detailed protocols for ChIP-seq and MethylCap-seq procedures applied to zebrafish (Danio rerio) embryonic material at four developmental stages. As a proof of principle, we have profiled on a global scale a number of post-translational histone modifications including H3K4me1, H3K4me3 and H3K27ac. We demonstrate that these marks are dynamic during early development and that such developmental transitions can be detected by ChIP-seq. In addition, we applied MethylCap-seq to show that developmentally-regulated DNA methylation remodeling can be detected by such a procedure. Our MethylCap-seq data concur with previous DNA methylation studies of early zebrafish development rendering this method highly suitable for the global assessment of DNA methylation in early vertebrate embryos.es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.rightsclosedAccesses_ES
dc.subjectEpigenomicses_ES
dc.subjectZebrafishes_ES
dc.subjectHistone modificationses_ES
dc.subjectDNA methylationes_ES
dc.subjectDevelopmentes_ES
dc.titleThe developmental epigenomics toolbox: ChIP-seq and MethylCap-seq profiling of early zebrafish embryoses_ES
dc.typeartículoes_ES
dc.identifier.doi10.1016/j.ymeth.2013.04.011-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.ymeth.2013.04.011es_ES
dc.identifier.e-issn1095-9130-
dc.relation.csices_ES
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