English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/129114
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Step-wise methylation of histone H3K9 positions heterochromatin at the nuclear periphery

AutorTowbin, Benjamin D.; González-Aguilera, Cristina ; Sack, Ragna; Gaidatzis, Dimos; Kalck, Véronique; Meister, Peter; Askjaer, Peter ; Gasser, Susan M.
Fecha de publicación2012
EditorElsevier
CitaciónCell 150(5): 934-947 (2012)
ResumenThe factors that sequester transcriptionally repressed heterochromatin at the nuclear periphery are currently unknown. In a genome-wide RNAi screen, we found that depletion of S-adenosylmethionine (SAM) synthetase reduces histone methylation globally and causes derepression and release of heterochromatin from the nuclear periphery in Caenorhabditis elegans embryos. Analysis of histone methyltransferases (HMTs) showed that elimination of two HMTs, MET-2 and SET-25, mimics the loss of SAM synthetase, abrogating the perinuclear attachment of heterochromatic transgenes and of native chromosomal arms rich in histone H3 lysine 9 methylation. The two HMTs target H3K9 in a consecutive fashion: MET-2, a SETDB1 homolog, mediates mono- and dimethylation, and SET-25, a previously uncharacterized HMT, deposits H3K9me3. SET-25 colocalizes with its own product in perinuclear foci, in a manner dependent on H3K9me3, but not on its catalytic domain. This colocalization suggests an autonomous, self-reinforcing mechanism for the establishment and propagation of repeat-rich heterochromatin.
Versión del editorhttp://dx.doi.org/10.1016/j.cell.2012.06.051
URIhttp://hdl.handle.net/10261/129114
DOI10.1016/j.cell.2012.06.051
ISSN0092-8674
E-ISSN1097-4172
Aparece en las colecciones: (CABD) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Step-wise methylation.pdf1,71 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.