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dc.contributor.authorMedina, Carloses_ES
dc.contributor.authorSantero, Eduardoes_ES
dc.contributor.authorGómez-Skarmeta, José Luises_ES
dc.contributor.authorRoyo, José Luises_ES
dc.date.accessioned2016-02-15T14:35:24Z-
dc.date.available2016-02-15T14:35:24Z-
dc.date.issued2012-
dc.identifier.citationJournal of Biotechnology 157(3): 413-416 (2012)es_ES
dc.identifier.issn0168-1656-
dc.identifier.urihttp://hdl.handle.net/10261/129100-
dc.descriptionShort communication.-
dc.description.abstractMicrobial host–pathogen interactions have been traditionally well studied at genetic and physiological levels, but cell-resolution analyses have been particularly scarce. This has been especially remarkable for intracellular parasites for two major reasons: first, the inherent loss of bacteria traceability once infects its hosts; second and more important, the limited availability of genetic tools that allow a tight regulated expression of bacterial virulence genes once inside the host tissues. Here we present novel data supporting the use of zebrafish embryos to monitor Salmonella enterica serovar Thyphimurium infection. Intravenous infection of Salmonella can be easily monitored using in vivo fluorescence that allows the visualization of free-swimming bacteria through the circulatory system. Moreover, we have engineered Salmonella to voluntarily activate heterologous gene expression at any point during infection once inside the zebrafish macrophages using a salicylate-based expression system. This approach allows real-time cell-resolution in vivo monitoring of the infection. All together, this approach paves the road to cell-based resolution experiments that would be harder to mimic in other vertebrate infection models.es_ES
dc.description.sponsorshiphis work was funded by grants BFU2010-14839, CSD2007-00008, CSD2007-00005 and Proyectos de Excelencia CVI-3488 and P07-CVI-02518 from the Spanish and Andalusian Governments, respectively. Jose Luis Royo holds a JAE DOC contract from the Spanish National Research Council (CSIC).es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relation.isversionofPostprint-
dc.rightsopenAccesses_ES
dc.subjectIn vivo protein expressiones_ES
dc.subjectSalmonella pathogenesises_ES
dc.subjectZebrafishes_ES
dc.titleEngineered Salmonella allows real-time heterologous gene expression monitoring within infected zebrafish embryoses_ES
dc.typeartículoes_ES
dc.identifier.doihttp://dx.doi.org/10.1016/j.jbiotec.2011.11.022-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.jbiotec.2011.11.022es_ES
dc.identifier.e-issn1873-4863-
dc.contributor.funderJunta de Andalucíaes_ES
dc.contributor.funderConsejo Superior de Investigaciones Científicas (España)es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.relation.csices_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003339es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
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