English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/129099
Título

Dynamics of enhancer chromatin signatures mark the transition from pluripotency to cell specification during embryogenesis

AutorBogdanovic, Ozren ; Fernández-Miñán, Ana ; Tena, Juan J. ; Calle-Mustienes, Elisa de la ; Hidalgo, Carmen; Veenstra, Gert Jan C.; Gómez-Skarmeta, José Luis
Fecha de publicación2012
EditorCold Spring Harbor Laboratory. Press
CitaciónGenome Research 22(10): 2043-2053 (2012)
ResumenThe generation of distinctive cell types that form different tissues and organs requires precise, temporal and spatial control of gene expression. This depends on specific cis-regulatory elements distributed in the noncoding DNA surrounding their target genes. Studies performed on mammalian embryonic stem cells and Drosophila embryos suggest that active enhancers form part of a defined chromatin landscape marked by histone H3 lysine 4 mono-methylation (H3K4me1) and histone H3 lysine 27 acetylation (H3K27ac). Nevertheless, little is known about the dynamics and the potential roles of these marks during vertebrate embryogenesis. Here, we provide genomic maps of H3K4me1/me3 and H3K27ac at four developmental time-points of zebrafish embryogenesis and analyze embryonic enhancer activity. We find that (1) changes in H3K27ac enrichment at enhancers accompany the shift from pluripotency to tissue-specific gene expression, (2) in early embryos, the peaks of H3K27ac enrichment are bound by pluripotent factors such as Nanog, and (3) the degree of evolutionary conservation is higher for enhancers that become marked by H3K27ac at the end of gastrulation, suggesting their implication in the establishment of the most conserved (phylotypic) transcriptome that is known to occur later at the pharyngula stage.
DescripciónThis article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date; after six months, it is available under a Creative Commons License.-- et al.
Versión del editorhttp://dx.doi.org/10.1101/gr.134833.111
URIhttp://hdl.handle.net/10261/129099
DOI10.1101/gr.134833.111
ISSN1088-9051
E-ISSN1549-5469
Aparece en las colecciones: (CABD) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
embryogenesis.pdf2,07 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.