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dc.contributor.authorIrimia, Manuel-
dc.contributor.authorRoyo, José Luis-
dc.contributor.authorBurguera, Demian-
dc.contributor.authorMaeso, Ignacio-
dc.contributor.authorGómez-Skarmeta, José Luis-
dc.contributor.authorGarcia-Fernàndez, Jordi-
dc.date.accessioned2016-02-15T10:36:57Z-
dc.date.available2016-02-15T10:36:57Z-
dc.date.issued2012-
dc.identifierdoi: 10.1038/srep00433-
dc.identifierissn: 2045-2322-
dc.identifier.citationScientific Reports 2: 433 (2012)-
dc.identifier.urihttp://hdl.handle.net/10261/129079-
dc.descriptionThis work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License.-
dc.description.abstractThe origin and evolution of the complex regulatory landscapes of some vertebrate developmental genes, often spanning hundreds of Kbp and including neighboring genes, remain poorly understood. The Sonic Hedgehog (Shh) genomic regulatory block (GRB) is one of the best functionally characterized examples, with several discrete enhancers reported within its introns, vast upstream gene-free region and neighboring genes (Lmbr1 and Rnf32). To investigate the origin and evolution of this GRB, we sequenced and characterized the Hedgehog (Hh) loci from three invertebrate chordate amphioxus species, which share several early expression domains with Shh. Using phylogenetic footprinting within and between chordate lineages, and reporter assays in zebrafish probing >30 Kbp of amphioxus Hh, we report large sequence and functional divergence between both groups. In addition, we show that the linkage of Shh to Lmbr1 and Rnf32, necessary for the unique gnatostomate-specific Shh limb expression, is a vertebrate novelty occurred between the two whole-genome duplications.-
dc.description.sponsorshipM.I., D.B., I.M. and J.G.-F. were funded by Grants BFU2005-00252 and BMC2008-03776 and BMC2011-23291 from the Spanish Ministry of Science and Innovation, and J.G.F. by the ICREA Academia Prize. M.I., D.B. and I.M. held FPI, APIF-UB and FPU fellowships, respectively. J.-L.G.-S. and J.L.R. were supported by Grants BFU2010-14839, CSD2007-00008875 (MEC), and CVI 3488 (Junta de Andalucía). JLR holds a JAE-Doc grant from the National Research Council, founded by Social European Funds.-
dc.publisherNature Publishing Group-
dc.relation.isversionofPublisher's version-
dc.rightsopenAccess-
dc.titleComparative genomics of the Hedgehog loci in chordates and the origins of Shh regulatory novelties-
dc.typeartículo-
dc.identifier.doi10.1038/srep00433-
dc.relation.publisherversionhttp://dx.doi.org/10.1038/srep00433-
dc.date.updated2016-02-15T10:36:59Z-
dc.description.versionPeer Reviewed-
dc.language.rfc3066eng-
dc.rights.licensehttp://creativecommons.org/licenses/by-nc-sa/3.0/-
dc.contributor.funderConsejo Superior de Investigaciones Científicas (España)-
dc.contributor.funderEuropean Commission-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
dc.contributor.funderMinisterio de Ciencia e Innovación (España)-
dc.contributor.funderInstitución Catalana de Investigación y Estudios Avanzados-
dc.contributor.funderJunta de Andalucía-
dc.relation.csic-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003339es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100004837es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003741es_ES
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