English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/129076
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Insight into the retention processes of phthalate metabolites on different liquid chromatography stationary phases for the development of improved separation methods

AuthorsGómara, B. ; Lebrón-Aguilar, Rosa ; González, María José; Quintanilla-López, Jesús Eduardo
KeywordsPhthalate metabolites
Liquid chromatography–mass spectrometry
Polar embedded stationary phases
Experimental design
Issue Date4-Dec-2015
PublisherElsevier
CitationJournal of Chromatography A 1423: 86-95 (2015)
AbstractThe retention behavior of nine MPAEs has been studied, using commercial LC columns with octadecylsilane (ODS), phenyl, and amide-type SPs. First, it was found that the use of methanol in the mobile phase is not advisable, because induce a transesterification reaction of MPAEs in the electrospray ion source, regardless of the SP used. On the other hand, different responses were observed when representing the logarithm of retention factors (k) vs. the volume fraction of ACN (φ) in the mobile phase, for the three SPs tested. A quite linear trend was obtained for ODS (at φ values below 0.80) and Phenyl columns. On the contrary, the Amide column shows a striking U-shape trend, typical of both hydrophobic and hydrophilic retention mechanisms. Therefore, the separation process was mainly hydrophobic in the ODS and phenyl SPs, but in the amide-type a dual retention mechanism was found, showing zones with predominant hydrophobic or hydrophilic interactions, depending on both the compound and the experimental conditions. A high content of acetonitrile (>75%) and low concentration of formic acid in the mobile phase promote the hydrophilic separation mechanism for MPAEs on the amide SP. So, this dual separation mechanism can be modulated modifying the pH and content of organic modifier in the mobile phase, allowing greater flexibility to develop improved methods. Taking advantage of this, a separation method was optimized in this amide column using a Box–Wilson Central Composite experimental design, which allows separating the studied MPAEs with a time-saving of around 40% comparing to the conventional phenyl SP.
Publisher version (URL)http://dx.doi.org/10.1016/j.chroma.2015.10.069
URIhttp://hdl.handle.net/10261/129076
DOI10.1016/j.chroma.2015.10.069
ISSN0021-9673
E-ISSN1873-3778
Appears in Collections:(IQOG) Artículos
(IQFR) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf14,54 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.