Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/129051
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Platero, Ana Isabel | - |
dc.contributor.author | García-Jaramillo, Manuel | - |
dc.contributor.author | Santero, Eduardo | - |
dc.contributor.author | Govantes, Fernando | - |
dc.date.accessioned | 2016-02-12T12:13:41Z | - |
dc.date.available | 2016-02-12T12:13:41Z | - |
dc.date.issued | 2012 | - |
dc.identifier | doi: 10.1128/JB.01348-12 | - |
dc.identifier | issn: 0021-9193 | - |
dc.identifier | e-issn: 1098-5530 | - |
dc.identifier.citation | Journal of Bacteriology 194(23): 6560-6573 (2012) | - |
dc.identifier.uri | http://hdl.handle.net/10261/129051 | - |
dc.description.abstract | The atzS-atzT-atzU-atzV-atzW gene cluster of the Pseudomonas sp. strain ADP atrazine-degradative plasmid pADP-1, which carries genes for an outer membrane protein and the components of a putative ABC-type solute transporter, is locateddownstream from atzR, which encodes the LysR-type transcriptional regulator of the cyanuric acid-degradative operon atzDEF. Here we describe the transcriptional organization of these genes. Our results show that all six genes are cotranscribed from the PatzR promoter to form the atzRSTUVW operon. A second, stronger promoter, PatzT, is found within atzS and directs transcription of the four distal genes. PatzT is σN dependent, activated by NtrC in response to nitrogen limitation with the aid of IHF, and repressed by AtzR. A combination of in vivo mutational analysis and primer extension allowed us to locate the PatzT promoter and map the transcriptional start site. Similarly, we used deletion and point mutationanalyses, along with in vivo expression studies and in vitro binding assays, to locate the NtrC, IHF, and AtzR binding sites and address their functionality. Our results suggest a regulatory model in which NtrC activates PatzT transcriptionvia DNA looping, while AtzR acts as an antiactivator that diminishes expression by interfering with the activation process. | - |
dc.description.sponsorship | Work in our lab is supported by grants BIO2004-01354, BIO2007-63754, BIO2010-17853, CSD2007-0005, and BIO2011-24003, cofunded by the Spanish Ministerio de Educación y Ciencia and the European Regional Development Fund. | - |
dc.publisher | American Society for Microbiology | - |
dc.rights | closedAccess | - |
dc.title | Transcriptional organization and regulatory elements of a Pseudomonas sp. strain ADP operon encoding a LysR-type regulator and a putative solute transport system | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1128/JB.01348-12 | - |
dc.date.updated | 2016-02-12T12:13:41Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.contributor.funder | Ministerio de Educación y Ciencia (España) | - |
dc.contributor.funder | European Commission | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.pmid | 23042989 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.fulltext | No Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.openairetype | artículo | - |
Aparece en las colecciones: | (CABD) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
5
checked on 26-mar-2024
SCOPUSTM
Citations
16
checked on 16-abr-2024
WEB OF SCIENCETM
Citations
16
checked on 29-feb-2024
Page view(s)
193
checked on 24-abr-2024
Download(s)
80
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.