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DNA Topology and Global Architecture of Point Centromeres

AutorDíaz-Ingelmo, Ofelia; Martínez-García, Belén; Segura, Joana; Valdés, Antonio; Roca, Joaquim
Fecha de publicación27-oct-2015
CitaciónCell Reports 13(4): 667-677 (2015)
ResumenDNA is wrapped in a left-handed fashion around histone octasomes containing the centromeric histone H3 variant CENP-A. However, DNA topology studies have suggested that DNA is wrapped in a righthanded manner around the CENP-A nucleosome that occupies the yeast point centromere. Here, we determine the DNA linking number difference (DLk) stabilized by the yeast centromere and the contribution of the centromere determining elements (CDEI, CDEII, and CDEIII). We show that the intrinsic architecture of the yeast centromere stabilizes +0.6 units of DLk. This topology depends on the integrity of CDEII and CDEIII, but it is independent of cbf1 binding to CDEI and of the variable length of CDEII. These findings suggest that the interaction of the CBF3 complex with CDEIII and a distal CDEII segment configures a right-handed DNA loop that excludes CDEI. This loop is then occupied by a CENP-A histone complex, which does not have to be inherently right-handed.
Versión del editorhttp://dx.doi.org/10.1016/j.celrep.2015.09.039
Identificadoresdoi: 10.1016/j.celrep.2015.09.039
issn: 2211-1247
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