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Comprenhesive characterization of goat colostrum oligosacharides by liquid chomatography coupled to mass spectrometry

AuthorsMartín-Ortiz, Andrea; Barile, Daniela; Salcedo, J.; Bunyatratchata, A.; Moreno, F. Javier CSIC ORCID ; Martín-García, I.; Clemente, Alfonso; Ruiz-Matute, Ana I. CSIC ORCID ; Sanz, M. Luz CSIC ORCID
Issue DateOct-2015
CitationXV SECyTA (2015)
VII SEEM (2015)
AbstractGoat milk is a complex mixture of nutritive and bioactive components with reported health benefits such as carbo-hydrates, lipids and proteins. Although lactose is the main carbohydrate, presence of other oligosaccharides (OS) similar to those found in human milk, has been reported. Some of these oligosaccharides, such as those containing fucosyl- or sialyl- groups have been described to have prebiotic and pathogen binding activities. In recent years, different analytical techniques have demonstrated their potential in OS characterization. Nanoflow Liquid Chromatography-Quadrupole-TOF MS (Nano-LC-Chip-Q-TOF MS) shows a high sensitivity and capacity for OS compositional verification, whereas, Hydrophilic Interaction Liquid Chromatography (HILIC) is a powerful LC operation mode for quantitative analysis of OS, providing appropriate resolution and good peak shapes. In this work, qualitative and quantitative analysis of goat colostrum oligosaccharides (GCO) has been carried out. Milk colostra, previously defatted and deproteinized, were treated by Size Exclusion Chromatography (SEC) to remove lactose. Up to 78 oligosaccharides containing hexose, hexosamine, fucose, N-acetylneuraminic acid or N-glycolylneuraminic acid monomeric units were identified in the samples by Nano-LC-Chip-Q-TOF MS, some of them detected for the first time in goat colostra. As a second step, a previously optimized HILIC-MS method was used for the separation and quantitation of the main GCO, both acidic and neutral compounds. Values ranging from 140 to 315 mg L-1 for neutral oligosaccharides and from 83 to 251 mg L-1 for acidic oligosaccharides were found. The most abundant OS were galactosyl-lactoses (124.92-265.77 mg L-1), followed by 6’-sialyl-lactoseand sialyl-lactosamine. To the best of our knowledge, this is the first time that a comprehensive characterization of GCO is carried out.
DescriptionResumen del póster presentado a la XV Reunión Científica de la Sociedad Española de Cromatografía y Técnicas Afines (SECyTA) y la VII Reunión Nacional de la Sociedad Española de Espectrometría de Masas (SEEM); celebradas en Castellón del 27 al 30 de octubre de 2015.
Appears in Collections:(CIAL) Comunicaciones congresos
(IQOG) Comunicaciones congresos
(EEZ) Comunicaciones congresos
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