English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/128899
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

Levels and temporal trends of phthalate metabolites in human Spanish urine samples from 2012 to 2015

AutorFernández Martín, Mario Antonio ; Herrero, Laura ; Quintanilla-López, Jesús Eduardo ; Calvarro, Sagrario ; Gómara, Belén; González, María José
Palabras clavePhthalates, metabolites, urine, humans
Fecha de publicaciónoct-2015
CitaciónXV Reunión Científica de la Sociedad Española de Cromatografía y Técnias Afines- SECyTA 2015
ResumenPhthalates (diesters of 1,2-benzenedicarboxylic acid), also known as phthalic acid esters (PAEs), are synthetic organic compounds that are used in a broad spectrum of industrial and commercial applications, like toys, food packaging, lubricants, vinyl flooring, adhesives, detergents, hair spray, shampoo, etc. [1] . They have endocrine disrupting potency and have become nearly ubiquitous. For the general population, the human exposure to phthalates is through ingestion, inhalation, dermal absorption, and/or contact with medical devices [2]. Once they are ingested, PAEs are rapidly metabolized to their respective monoesters in human body and some of these primary metabolites can be further metabolized to secondary metabolites and excreted by urine. All of these metabolites can be used as biomarkers of human exposure to phthalates [3]. The sample preparation and instrumental determination methods have been previously described elsewhere [4]. Briefly, the sample preparation consisted of an enzymatic deconjugation of the glucuronide phthalates followed by a purification step with OASIS© HLB SPE cartridge (Waters, MA, USA). The instrumental determination was carried out by using UPLC QqQ MS2 (Xevo-TQS, Waters) working in MRM mode, with a BEH-phenyl column (50 mm x 2.1 mm, 1.7 µm),. We present here, for the first time, the levels and temporal trends of 9 PAE metabolites (6 phthalate monoesters (monomethyl phthalate [MMP], monoethyl phthalate [MEP], mono-iso-buthyl phthalate [MiBP], mono-n-butyl phthalate [MBP], monobenzyl phthalate [MBzP] and monoethylhexyl phthalate [MEHP]) and 3 secondary metabolites of DEHP (5-OH-mono(2-ethylhexyl) phthalate [5-OH-MEHP], 5-oxo-mono(2-ethylhexyl) phthalate [5oxo-MEHP] and 5-carboxy-mono(2-ethylpentyl) phthalate [5cx-MEPP]) in human urine samples collected in 2012 and 2015 from 9 volunteers living in the Community of Madrid. Detectable amounts of the 9 MPAEs were obtained in all the studied samples, being the MPAE levels found in almost all collected in 2012 slightly lower than those found three years later (2015). [1] T. Schettler. Int. J. Androl. 29 (2006) 134-139. [2] R. Hauser, A. Calafat. Occup. Environ. Med. 62 (2005) 806–818. [3] H. Frederiksen, S.K. Kranich. Environ. Sci. Technol. 47 (2013) 958–967. [4] L. Herrero, S. Calvarro. Anal Chim Acta 853 (2015) 625–636. Acknowledgements: Authors wish to thank all the volunteers who have donated samples and Paula Chamorro and Héctor Carrascosa for their laboratory work. Financial support was obtained from the Spanish Ministry of Economy and Competitiveness (project AGL2012-37201) and the Comunidad Autónoma of Madrid and European funding from FEDER program (projects S2009/AGR-1464, ANALISYC-II and S2013/ABI-3028, AVANSECAL).
DescripciónTrabajo presentado a la XV Reunión científica de la Sociedad Española de Cromatografía y Técnicas Afines - SECyTA 2015. Castellón de la Plana, 27-30 de octubre de 2015.
URIhttp://hdl.handle.net/10261/128899
Aparece en las colecciones: (IQOG) Comunicaciones congresos
(IQFR) Comunicaciones congresos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Levels and temporal trends of phthalate.pdf243,47 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.