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Título

Presence of nitrogen-fixing microorganisms in seagrass, Posidonia oceanica, roots and nitrogen isotopic signature of seagrass tissues

AutorGarcias-Bonet, Neus; Arrieta López de Uralde, Jesús M. ; Duarte, Carlos M. ; Marbà, Núria
Palabras claveNitrogen isotopic signature
Seagrass
Posidonia oceanica
Nitrogen fixers
nifH gene
δ15N
Fecha de publicación9-feb-2016
CitaciónGarcias-Bonet, Neus; Arrieta López de Uralde, Jesús M.; Duarte, Carlos M.; Marbà, Núria, 2016, "Presence of nitrogen-fixing microorganisms in seagrass, Posidonia oceanica, roots and nitrogen isotopic signature of seagrass tissues [Dataset]", DIGITAL.CSIC, http://dx.doi.org/10.20350/digitalCSIC/226
ResumenThis dataset compiles information regarding the detection of nitrogen-fixing bacteria by amplification of the nifH gene coding for nitrogenase enzyme in meadows of an endemic Mediterranean seagrass (Posidonia oceanica). This dataset includes the nitrogen isotopic signature (δ15N) measured on leaves and rhizomes.
DescripciónTriplicate samples of Posidonia oceanica were randomly collected at 26 locations along the Balearic Islands (Mediterranean Sea) during the summers of 2005 and 2006. Roots were subjected to a surface-sterilization protocol prior to nucleic acid extraction. The nitrogenase was amplified by PCR using degenerate primers for nifH gene sequences. The PCR products were checked by electrophoresis on 1.5% agarose gels. The youngest leaf (free of epiphytes) of three shoots and three young rhizome fragments were dried at 60ºC for 48 h and ground to a fine powder. All isotopic analyses were measured using standard elemental analyzer isotope ratio mass spectrometer (EA-IRMS) procedures. Isotopic ratios (R) are reported in the standard delta notation (‰), deltasample=1000((Rsample/Rstandard)-1), where R = 15N/14N. These results are presented with respect to the International standard of atmospheric nitrogen (AIR, N2). Analytical reproducibility of the reported delta values, based on sample replicates, was better than ±0.2‰.
URIhttp://hdl.handle.net/10261/128883
DOIhttp://dx.doi.org/10.20350/digitalCSIC/226
ReferenciasUnderlying dataset of the following paper: Garcias-Bonet, Neus; Arrieta, Jesús M.; Duarte, Carlos M.; Marbà, Núria: Nitrogen-fixing bacteria in Mediterranean seagrass (Posidonia oceanica) roots 2016, DOI: 10.1016/j.aquabot.2016.03.002.
Aparece en las colecciones: (IMEDEA) Conjuntos de datos
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Dataset_DeltaN.csv1,79 kBUnknownVisualizar/Abrir
Dataset_DeltaN.xlsx12,35 kBMicrosoft Excel XMLVisualizar/Abrir
Variables_Dataset_delta15N.xlsx8,99 kBMicrosoft Excel XMLVisualizar/Abrir
ReadMe__Delta_dataset.txt1,3 kBTextVisualizar/Abrir
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