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Compositional changes induced by UV-B radiation treatment of common bean and soybean seedlings monitored by capillary electrophoresis with diode array detection

AutorDinelli, Giovanni; Aloisio, Irene; Bonetti, Alessandra; Marotti, Ilaria; Cifuentes, Alejandro
Palabras clavebean sprouts
food analysis
UV treatments
Fecha de publicaciónmar-2007
CitaciónJ. Sep. Sci. 30 (2007) 604-611
ResumenIn this work, a new capillary electrophoresis (CE) method with diode array detection (DAD) was developed for the monitoring and quantitation of flavonoids in different beans treated and untreated with UV-B radiation. Namely, flavonoid concentration was monitored in UV-B treated and untreated sprouts of three common beans (Zolfino ecotype, cv. Verdone, cv. Lingua di Fuoco) and one soybean (cv. Pacific). After acid hydrolysis of extracts, the CE-DAD method provides reproducible quantitative determinations of daidzein, glycitein, genistein and kaempferol at ppm level in these natural matrices within a relatively short time (less than 16 min). Total flavonoid content determined by CE-DAD was 159 ± 8, 26 ± 2, 13 ± 1, 1.3 ± 0.3 µg/g fresh weight for untreated sprouts of Pacific soybean, Verdone bean, Zolfino bean and Lingua di Fuoco bean, respectively. UV-B treatment caused no significant quantitative effect on Pacific soybean sprouts, whereas it enhanced total isoflavone content 1.5, 1.8 and 3.2-fold in Verdone, Zolfino and Lingua di Fuoco beans, respectively. The proposed method shows i) the potentialities of bean sprouts as a natural source of bioactive compounds (antioxidants); ii) the technological role of UV-B treatment for sprout isoflavone enrichment and iii) the good capabilities of CE-DAD to monitor this process.
Versión del editor10.1002/jssc.200600289
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