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Título

Endothelium-derived microparticles from chronically thromboembolic pulmonary hypertensive patients facilitate endothelial angiogenesis

AutorBelik, Daria; Tsang, Hilda; Wharton, John; Howard, Luke; Bernabéu, Carmelo ; Wojciak-Stothard, Beata
Palabras claveEndoglin
Angiogenesis
Pulmonary hypertension
Microparticles
Fecha de publicación19-ene-2016
EditorBioMed Central
CitaciónJournal of Biomedical Science 23:4 (2016)
ResumenBackground: Increased circulating levels of endoglin+ endothelial microparticles (EMPs) have been identified in several cardiovascular disorders, related to severity. Endoglin is an auxilary receptor for transforming growth factor β (TGF-β) important in the regulation of vascular structure.
Results: We quantified the number of microparticles in plasma of six patients with chronic thromboembolic pulmonary hypertension (CTEPH) and age- and sex-matched pulmonary embolic (PE) and healthy controls and investigated the role of microparticle endoglin in the regulation of pulmonary endothelial function in vitro. Results show significantly increased levels of endoglin+ EMPs in CTEPH plasma, compared to healthy and disease controls. Co-culture of human pulmonary endothelial cells with CTEPH microparticles increased intracellular levels of endoglin and enhanced TGF-β-induced angiogenesis and Smad1,5,8 phosphorylation in cells, without affecting BMPRII expression. In an in vitro model, we generated endothelium-derived MPs with enforced membrane localization of endoglin. Co-culture of these MPs with endothelial cells increased cellular endoglin content, improved cell survival and stimulated angiogenesis in a manner similar to the effects induced by overexpressed protein.
Conclusions: Increased generation of endoglin+ EMPs in CTEPH is likely to represent a protective mechanism supporting endothelial cell survival and angiogenesis, set to counteract the effects of vascular occlusion and endothelial damage.
Descripción11 p.-4 fig.-1 tab.
Versión del editorhttp://dx.doi.org/ 10.1186/s12929-016-0224-9
URIhttp://hdl.handle.net/10261/128767
DOI10.1186/s12929-016-0224-9
ISSN1021-7770
E-ISSN1423-0127
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