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Suppression of spermatogenesis before grafting increases survival and supports resurgence of spermatogenesis in adult mouse testis

AutorArregui, Lucía; Rathi, Rahul; Modelski, Mark; Zeng, Wenxian; Roldán, Eduardo R. S. ; Dobrinski, Ina
Palabras claveCryptorchidism
Fecha de publicación30-mar-2012
CitaciónFertility and Sterility 97(6): 1422-1429 (2012)
ResumenObjective: To test whether absence of complete spermatogenesis in mature testicular tissue before grafting will increase graft survival. Design: Prospective experimental study. Setting: Laboratory. Animal(s): Donor testes were obtained from adult untreated mice, adult mice rendered cryptorchid, and adult mice treated with a GnRH antagonist (acyline). Intervention(s): Donor testes were ectopically grafted to nude mice and recovered at three time points. Main Outcome Measure(s): Most advanced germ cell type and presence of spermatogonia were assessed. Donor testes and grafts were analyzed by histology and by immunocytochemistry for ubiquitin C-terminal hydrolase-L1 to mark germ cells. Result(s): Suppression of spermatogenesis by inducing cryptorchidism or acyline treatment resulted in improved survival of grafted tissue compared with controls and recovery of complete spermatogenesis, whereas control testis grafts mostly degenerated and did not restore complete spermatogenesis. Conclusion(s): These results indicate that complete spermatogenesis at the time of grafting has a negative effect on graft survival. Grafting of adult testis tissue from donors with suppressed spermatogenesis leads to spermatogenic recovery and may provide a tool to study and preserve fertility and for conservation of genetic resources in individuals that lack complete germ cell differentiation. © 2012 American Society for Reproductive Medicine.
Identificadoresdoi: 10.1016/j.fertnstert.2012.03.009
issn: 0015-0282
e-issn: 1556-5653
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