English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/128742
Título

Suppression of spermatogenesis before grafting increases survival and supports resurgence of spermatogenesis in adult mouse testis

AutorArregui, Lucía; Rathi, Rahul; Modelski, Mark; Zeng, Wenxian; Roldán, Eduardo R. S. ; Dobrinski, Ina
Palabras claveCryptorchidism
Spermatogenesis
Acyline
Grafting
Fecha de publicación30-mar-2012
EditorElsevier
CitaciónFertility and Sterility 97(6): 1422-1429 (2012)
ResumenObjective: To test whether absence of complete spermatogenesis in mature testicular tissue before grafting will increase graft survival. Design: Prospective experimental study. Setting: Laboratory. Animal(s): Donor testes were obtained from adult untreated mice, adult mice rendered cryptorchid, and adult mice treated with a GnRH antagonist (acyline). Intervention(s): Donor testes were ectopically grafted to nude mice and recovered at three time points. Main Outcome Measure(s): Most advanced germ cell type and presence of spermatogonia were assessed. Donor testes and grafts were analyzed by histology and by immunocytochemistry for ubiquitin C-terminal hydrolase-L1 to mark germ cells. Result(s): Suppression of spermatogenesis by inducing cryptorchidism or acyline treatment resulted in improved survival of grafted tissue compared with controls and recovery of complete spermatogenesis, whereas control testis grafts mostly degenerated and did not restore complete spermatogenesis. Conclusion(s): These results indicate that complete spermatogenesis at the time of grafting has a negative effect on graft survival. Grafting of adult testis tissue from donors with suppressed spermatogenesis leads to spermatogenic recovery and may provide a tool to study and preserve fertility and for conservation of genetic resources in individuals that lack complete germ cell differentiation. © 2012 American Society for Reproductive Medicine.
URIhttp://hdl.handle.net/10261/128742
DOI10.1016/j.fertnstert.2012.03.009
Identificadoresdoi: 10.1016/j.fertnstert.2012.03.009
issn: 0015-0282
e-issn: 1556-5653
Aparece en las colecciones: (MNCN) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Fertil Steril. 2012 Jun 97(6) 1422–1429.pdf1,44 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.