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Secretory phospholipase A2 group IID is involved in progesterone-induced acrosomal exocytosis of human spermatozoa

AutorLi, Kun.; Jin, Jian-Yuan; Chen, Wen-Ying; Shi, Qi-Xian; Ni, Ya; Roldán, Eduardo R. S.
Palabras clavesPLA2-IID
Hyperactivation
Progesterone
Sperm
Fecha de publicación12-ene-2012
EditorJohn Wiley & Sons
American Society of Andrology
CitaciónJournal of Andrology 33(5): 975-983 (2012)
ResumenPhospholipase A2 (PLA2) plays a major role during acrosomal exocytosis (AE) in mammalian spermatozoa, but the identity of PLA2 subtypes present in spermatozoa remains elusive. This study explored whether secretory PLA2 Group IID (sPLA2-IID) isoform is present in human spermatozoa and whether it is involved in AE. Localization and expression of sPLA2-IID in human spermatozoa were explored by immunofluorescence staining and Western blot analysis. Occurrence of AE was evaluated by triple staining, and arachidonic acid (AA) levels were quantified by gas chromatography- mass spectrometry. Sperm motion parameters and hyperactivation were analyzed by computer-assisted sperm analysis. sPLA2-IID was localized in the postacrosomal region of the head and the midpiece of tail in human sperm. A 16-kd protein band was detected by Western blotting in sperm extracts. Progesterone-induced AE was significantly inhibited in a concentration-dependent manner using a sPLA2-IID neutralizing antibody. The increase in AA levels seen during progesterone-stimulated exocytosis was significantly abrogated by the antibody. The sPLA2-IID antibody significantly inhibited hyperactivation, sperm curvilinear velocity, and amplitude of lateral head displacement, but it did not affect the proportion of motile sperm. In conclusion, sPLA2-IID is present at the head and midpiece in the human sperm, and activation of such sPLA2-IID seems to be involved in AE. Therefore, sPLA2-IID isoform plays a functional role during the AE in human sperm. © American Society of Andrology.
URIhttp://hdl.handle.net/10261/128737
DOI10.2164/jandrol.111.014886
Identificadoresdoi: 10.2164/jandrol.111.014886
issn: 0196-3635
e-issn: 1939-4640
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