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dc.contributor.authorFernández-López, Raules_ES
dc.contributor.authorRuiz, Raúles_ES
dc.contributor.authorCruz, Fernando de laes_ES
dc.contributor.authorMoncalián, Gabrieles_ES
dc.date.accessioned2016-02-02T08:10:20Z-
dc.date.available2016-02-02T08:10:20Z-
dc.date.issued2015-07-01-
dc.identifier.citationFrontiers in Microbiology 6: 648 (2015)es_ES
dc.identifier.issn1664-302X-
dc.identifier.urihttp://hdl.handle.net/10261/128481-
dc.description.abstractWhole cell biosensors (WCBs) have multiple applications for environmental monitoring, detecting a wide range of pollutants. WCBs depend critically on the sensitivity and specificity of the transcription factor (TF) used to detect the analyte. We describe the mechanism of regulation and the structural and biochemical properties of TF families that are used, or could be used, for the development of environmental WCBs. Focusing on the chemical nature of the analyte, we review TFs that respond to aromatic compounds (XylS-AraC, XylR-NtrC, and LysR), metal ions (MerR, ArsR, DtxR, Fur, and NikR) or antibiotics (TetR and MarR). Analyzing the structural domains involved in DNA recognition, we highlight the similitudes in the DNA binding domains (DBDs) of these TF families. Opposite to DBDs, the wide range of analytes detected by TFs results in a diversity of structures at the effector binding domain. The modular architecture of TFs opens the possibility of engineering TFs with hybrid DNA and effector specificities. Yet, the lack of a crisp correlation between structural domains and specific functions makes this a challenging task.es_ES
dc.description.sponsorshipWe are grateful to Spanish Ministry of Economy for Grant BIO2010-14809 to GM. Work in FdlC laboratory was financed by the Spanish Ministry of Economy and Competitivity (BFU2011-26608) and by the European Seventh Framework Program [projects 612146/FP7-ICT-2013-10 (PLASWIRES), 289326/KBBE-2011-5 (ST-FLOW) and 282004/FP7-HEALTH-2011-2.3.1-2 (EvoTAR)].es_ES
dc.description.sponsorshipUSD 1,425 APC fee funded by the EC FP7 Post-Grant Open Access Pilotes_ES
dc.language.isoenges_ES
dc.publisherFrontiers in Bioscience Publicationses_ES
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/612146es_ES
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/289326es_ES
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/282004es_ES
dc.relationFP7 post-grant Gold Open Access Pilotes_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rightsopenAccesses_ES
dc.subjectBiosensorses_ES
dc.subjectTranscription factores_ES
dc.subjectEffectores_ES
dc.subjectAromatic compoundses_ES
dc.subjectMetales_ES
dc.subjectAnalytees_ES
dc.titleTranscription factor-based biosensors enlightened by the analytees_ES
dc.typeArtículoes_ES
dc.identifier.doi10.3389/fmicb.2015.00648-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.3389/fmicb.2015.00648-
dc.rights.licensehttp://creativecommons.org/licenses/by/4.0/es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderEuropean Commissiones_ES
dc.relation.csices_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
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