English
español
Please use this identifier to cite or link to this item:
http://hdl.handle.net/10261/128195
Share/Impact:
Statistics |
![]() ![]() ![]() |
|
|
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | |||
|
Title: | Pre-amyloid oligomers of the proteotoxic RepA-WH1 prionoid assemble at the bacterial nucleoid |
Authors: | Moreno-del Álamo, María ![]() ![]() ![]() ![]() |
Issue Date: | 1-Oct-2015 |
Publisher: | Nature Publishing Group |
Citation: | Scientific Reports 5: 14669 (2015) |
Abstract: | Upon binding to short specific dsDNA sequences in vitro, the N-terminal WH1 domain of the plasmid DNA replication initiator RepA assembles as amyloid fibres. These are bundles of single or double twisted tubular filaments in which distorted RepA-WH1 monomers are the building blocks. When expressed in Escherichia coli, RepA-WH1 triggers the first synthetic amyloid proteinopathy in bacteria, recapitulating some of the features of mammalian prion diseases: it is vertically transmissible, albeit non-infectious, showing up in at least two phenotypically distinct and interconvertible strains. Here we report B3h7, a monoclonal antibody specific for oligomers of RepA-WH1, but which does not recognize the mature amyloid fibres. Unlike a control polyclonal antibody generated against the soluble protein, B3h7 interferes in vitro with DNA-promoted or amyloid-seeded assembly of RepA-WH1 fibres, thus the targeted oligomers are on-pathway amyloidogenic intermediates. Immuno-electron microscopy with B3h7 on thin sections of E. coli cells expressing RepA-WH1 consistently labels the bacterial nucleoid, but not the large cytoplasmic aggregates of the protein. This observation points to the nucleoid as the place where oligomeric amyloid precursors of RepA-WH1 are generated, and suggests that, once nucleated by DNA, further growth must continue in the cytoplasm due to entropic exclusion. |
Description: | 12 p.-4 fig. |
Publisher version (URL): | http://dx.doi.org/ 10.1038/srep14669 |
URI: | http://hdl.handle.net/10261/128195 |
DOI: | 10.1038/srep14669 |
ISSN: | 2045-2322 |
E-ISSN: | 2045-2322 |
Appears in Collections: | (CIB) Artículos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
srep_Giraldo, R._2015.pdf | Artículo principal | 2,91 MB | Adobe PDF | ![]() View/Open |
Show full item record
Review this work
Review this work
Related articles:
WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.