English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/128180
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Selective constraints on protamine 2 in primates and rodents

AuthorsLüke, Lena; Tourmente, Maximiliano ; Dopazo, Hernán; Serra, François; Roldán, Eduardo R. S.
KeywordsProtamine
Evolution
Sexual selection
Sperm competition
Primates
Rodents
Spermatozoa
Issue Date22-Jan-2016
PublisherBioMed Central
CitationBMC Evolutionary Biology 16 : 21 (2016)
AbstractBackground: Protamines are sperm nuclear proteins with a crucial role in chromatin condensation. Their function is strongly linked to sperm head morphology and male fertility. Protamines appear to be affected by a complex pattern of selective constraints. Previous studies showed that sexual selection affects protamine coding sequence and expression in rodents. Here we analyze selective constraints and post-copulatory sexual selection acting on protamine 2 (Prm2) gene sequences of 53 species of primates and rodents. We focused on possible differences in selective constraints between these two clades and on the two functional domains of PRM2 (cleaved- and mature-PRM2). We also assessed if and how changes in Prm2 coding sequence may affect sperm head dimensions.
Results: The domain of Prm2 that is cleaved off during binding to DNA (cleaved-Prm2) was found to be under purifying selection in both clades, whereas the domain that remains bound to DNA (mature-Prm2) was found to be positively selected in primates and under relaxed constraint in rodents. Changes in cleaved-Prm2 coding sequence are significantly correlated to sperm head width and elongation in rodents. Contrary to expectations, a significant effect of sexual selection was not found on either domain or clade.
Conclusions: Mature-PRM2 may be free to evolve under less constraint due to the existence of PRM1 as a more conserved and functionally redundant copy. The cleaved-PRM2 domain seems to play an important role in sperm head shaping. However, sexual selection on its sequence may be difficult to detect until it is identified which sperm head phenotype (shape and size) confers advantages for sperm performance in different mammalian clades.
Publisher version (URL)http://dx.doi.org/10.1186/s12862-016-0588-1
URIhttp://hdl.handle.net/10261/128180
DOI10.1186/s12862-016-0588-1
E-ISSN1471-2148
Appears in Collections:(MNCN) Artículos
Files in This Item:
File Description SizeFormat 
BMC Evolutionary Biology (2016) 1621.pdf2,91 MBAdobe PDFThumbnail
View/Open