English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/127974
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

The development of genetic and molecular markers to register and commercialize Penicillium rubens (formerly Penicillium oxalicum) strain 212 as a biocontrol agent

AutorVillarino, María; De Cal, Antonieta; Melgarejo, P.; Larena, Inmaculada; Espeso, Eduardo A.
Fecha de publicación15-oct-2015
EditorJohn Wiley & Sons
CitaciónMicrobial Biotechnology 9(1), 89–99 (2016)
ResumenPenicillium oxalicum strain 212 (PO212) is an effective biocontrol agent (BCA) against a large number of economically important fungal plant pathogens. For successful registration as a BCA in Europe, PO212 must be accurately identified. In this report, we describe the use of classical genetic and molecular markers to characterize and identify PO212 in order to understand its ecological role in the environment or host. We successfully generated pyrimidine (pyr-) auxotrophic mutants. In addition we also designed specific oligonucleotides for the pyrF gene at their untranslated regions for rapid and reliable identification and classification of strains of P. oxalicum and P. rubens, formerly P. chrysogenum. Using these DNA-based technologies, we found that PO212 is a strain of P. rubens, and is not a strain of P. oxalicum. This work presents PO212 as the unique P. rubens strain to be described as a BCA and the information contained here serves for its registration and commercialization in Europe.
Descripción4 fig.-3 tab.
Versión del editorhttp://dx.doi.org/ 10.1111/1751-7915.12325
URIhttp://hdl.handle.net/10261/127974
DOI10.1111/1751-7915.12325
ISSN1751-7915
E-ISSN1751-7915
Aparece en las colecciones: (CIB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Villarino_et_al-2016-Microbial_Biotechnology.pdfArtículo principal2,46 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.