English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/127974
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

The development of genetic and molecular markers to register and commercialize Penicillium rubens (formerly Penicillium oxalicum) strain 212 as a biocontrol agent

AuthorsVillarino, María; De Cal, Antonieta; Melgarejo, P.; Larena, Inmaculada; Espeso, Eduardo A.
Issue Date15-Oct-2015
PublisherJohn Wiley & Sons
CitationMicrobial Biotechnology 9(1), 89–99 (2016)
AbstractPenicillium oxalicum strain 212 (PO212) is an effective biocontrol agent (BCA) against a large number of economically important fungal plant pathogens. For successful registration as a BCA in Europe, PO212 must be accurately identified. In this report, we describe the use of classical genetic and molecular markers to characterize and identify PO212 in order to understand its ecological role in the environment or host. We successfully generated pyrimidine (pyr-) auxotrophic mutants. In addition we also designed specific oligonucleotides for the pyrF gene at their untranslated regions for rapid and reliable identification and classification of strains of P. oxalicum and P. rubens, formerly P. chrysogenum. Using these DNA-based technologies, we found that PO212 is a strain of P. rubens, and is not a strain of P. oxalicum. This work presents PO212 as the unique P. rubens strain to be described as a BCA and the information contained here serves for its registration and commercialization in Europe.
Description4 fig.-3 tab.
Publisher version (URL)http://dx.doi.org/ 10.1111/1751-7915.12325
URIhttp://hdl.handle.net/10261/127974
DOI10.1111/1751-7915.12325
ISSN1751-7915
E-ISSN1751-7915
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
Villarino_et_al-2016-Microbial_Biotechnology.pdfArtículo principal2,46 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.