English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/127948
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Human nonsense-mediated mRNA decay factor UPF2 interacts directly with eRF3 and the SURF complex

AuthorsLópez-Perrote, Andrés ; Castaño, Raquel; Melero, Roberto ; Zamarro, María Teresa ; Llorca, Óscar
Issue Date5-Jan-2016
PublisherOxford University Press
CitationNucl. Acids Res. (2016)
AbstractNonsense-mediated mRNA decay (NMD) is an mRNA degradation pathway that regulates gene expression and mRNA quality. A complex network of macromolecular interactions regulates NMD initiation, which is only partially understood. According to prevailing models, NMD begins by the assembly of the SURF (SMG1–UPF1–eRF1–eRF3) complex at the ribosome, followed by UPF1 activation by additional factors such as UPF2 and UPF3. Elucidating the interactions between NMD factors is essential to comprehend NMD, and here we demonstrate biochemically and structurally the interaction between human UPF2 and eukaryotic release factor 3 (eRF3). In addition, we find that UPF2 associates with SURF and ribosomes in cells, in an UPF3 independent manner. Binding assays using a collection of UPF2 truncated variants reveal that eRF3 binds to the C-terminal part of UPF2. This region of UPF2 is partially coincident with the UPF3-binding site as revealed by electron microscopy of the UPF2–eRF3 complex. Accordingly, mwe find that the interaction of UPF2 with UPF3b interferes with the assembly of the UPF2–eRF3 complex, and that UPF2 binds UPF3b more strongly than eRF3.Together, our results highlight the role of UPF2 as a platform for the transient interactions of several NMD factors, including several components of SURF.
Description15 p.-6 fig.-1 tab. López Perrote, Andrés et al.
Publisher version (URL)http://dx.doi.org/ 10.1093/nar/gkv1527
URIhttp://hdl.handle.net/10261/127948
DOI10.1093/nar/gkv1527
ISSN0305-1048
E-ISSN1362-4962
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
Nucl. Acids Res.-2016-López-Perrote-nar-gkv1527.pdfArtículo principal5,22 MBAdobe PDFThumbnail
View/Open
nar-supp.dat.López-Perrote.pdfDatos suplementarios1,11 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.