English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/127780
Título

Gene Expression Analysis of Plum pox virus (Sharka) Susceptibility/Resistance in Apricot ( Prunus armeniaca L.)

AutorRubio, Manuel ; Ballester Frutos, Ana Rosa ; Olivares, Pedro M.; Castro de Moura, Manuel; Dicenta, Federico ; Martínez-Gómez, Pedro
Palabras claveRNA-Seq
Plum pox virus
Pathogen response
Infection
Resistence
Genomics
Fecha de publicación11-dic-2015
EditorPublic Library of Science
CitaciónPLoS ONE 10(12): e0144670 (2015)
ResumenRNA-Seq has proven to be a very powerful tool in the analysis of the Plum pox virus (PPV, sharka disease)/Prunus interaction. This technique is an important complementary tool to other means of studying genomics. In this work an analysis of gene expression of resistance/susceptibility to PPV in apricot is performed. RNA-Seq has been applied to analyse the gene expression changes induced by PPV infection in leaves from two full-sib apricot genotypes, “Rojo Pasión” and “Z506-7”, resistant and susceptible to PPV, respectively. Transcriptomic analyses revealed the existence of more than 2,000 genes related to the pathogen response and resistance to PPV in apricot. These results showed that the response to infection by the virus in the susceptible genotype is associated with an induction of genes involved in pathogen resistance such as the allene oxide synthase, S-adenosylmethionine synthetase 2 and the major MLP-like protein 423. Over-expression of the Dicer protein 2a may indicate the suppression of a gene silencing mechanism of the plant by PPV HCPro and P1 PPV proteins. On the other hand, there were 164 genes involved in resistance mechanisms that have been identified in apricot, 49 of which are located in the PPVres region (scaffold 1 positions from 8,050,804 to 8,244,925), which is responsible for PPV resistance in apricot. Among these genes in apricot there are several MATH domain-containing genes, although other genes inside (Pleiotropic drug resistance 9 gene) or outside (CAP, Cysteine-rich secretory proteins, Antigen 5 and Pathogenesis-related 1 protein; and LEA, Late embryogenesis abundant protein) PPVres region could also be involved in the resistance.
Versión del editorhttp://dx.doi.org/10.1371/journal.pone.0144670
URIhttp://hdl.handle.net/10261/127780
DOI10.1371/journal.pone.0144670
ISSN1932-620
Aparece en las colecciones: (IATA) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
pone-2015-Ballester.pdf2,03 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


Este item está licenciado bajo una Licencia Creative Commons Creative Commons