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Highly sensitive molecular diagnosis of prostate cancer using surplus material washed off from biopsy needles

AuthorsBermudo, Raquel; Abia, David ; Mozos, A.; García-Cruz, E.; Alcaraz, A.; Ortiz, Ángel R. ; Thomson, Timothy M. ; Fernández, Pedro L.
KeywordsLinear discriminant analysis
Gene signature
Needle biopsy
Prostate cancer
Real-time RT–PCR
Issue Date2011
PublisherNature Publishing Group
CitationBritish Journal of Cancer 105: 1600- 1607 (2011)
AbstractINTRODUCTION: Currently, final diagnosis of prostate cancer (PCa) is based on histopathological analysis of needle biopsies, but this process often bears uncertainties due to small sample size, tumour focality and pathologist’s subjective assessment. METHODS: Prostate cancer diagnostic signatures were generated by applying linear discriminant analysis to microarray and real-time RT–PCR (qRT–PCR) data from normal and tumoural prostate tissue samples. Additionally, after removal of biopsy tissues, material washed off from transrectal biopsy needles was used for molecular profiling and discriminant analysis. RESULTS: Linear discriminant analysis applied to microarray data for a set of 318 genes differentially expressed between non-tumoural and tumoural prostate samples produced 26 gene signatures, which classified the 84 samples used with 100% accuracy. To identify signatures potentially useful for the diagnosis of prostate biopsies, surplus material washed off from routine biopsy needles from 53 patients was used to generate qRT–PCR data for a subset of 11 genes. This analysis identified a six-gene signature that correctly assigned the biopsies as benign or tumoural in 92.6% of the cases, with 88.8% sensitivity and 96.1% specificity. CONCLUSION: Surplus material from prostate needle biopsies can be used for minimal-size gene signature analysis for sensitive and accurate discrimination between non-tumoural and tumoural prostates, without interference with current diagnostic procedures. This approach could be a useful adjunct to current procedures in PCa diagnosis. British Journal of Cancer (2011) 105, 1600–1607. doi:10.1038/bjc.2011.435 www.bjcancer.com Published online 18 October 2011 & 2011 Cancer Research UK
Publisher version (URL)http://dx.doi.org710.1038/bjc.2011.435
Identifiersdoi: 10.1038/bjc.2011.435
issn: 0007-0920
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