Please use this identifier to cite or link to this item:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Identification and mapping of quantitative trait loci for leaf rust resistance derived from a tetraploid wheat Triticum dicoccum accession

AuthorsDesiderio, Francesca; Guerra, Davide; Rubiales, Diego CSIC ORCID ; Piarulli, Luciana; Mastrangelo, Anna M.; Simeone, Rosanna; Blanco, Antonio M.; Cattivelli, Luigi; Vale’, G.
KeywordsTriticum turgidum ssp. dicoccum
High-density map
Molecular mapping
SNP marker
Leaf rust
Issue Date7-Nov-2014
PublisherKluwer Academic Publishers
CitationMolecular Breeding 34: 1659- 1675 (2014)
AbstractThe Triticum turgidum ssp. dicoccum (2n = 4x = 28) accession MG5323 showed a useful level of resistance to leaf rust disease. A segregating population of 110 recombinant inbred lines (RILs), derived from a cross between cv Latino (T. turgidum spp. durum), susceptible to leaf rust, and MG5323 was evaluated for reactions of seedlings to two different Puccinia triticina isolates. Genotyping of the RILs was performed with different molecular markers (SSR, EST-SSR and SNP), leading to the construction of a linkage map containing 10,840 loci covering 14 chromosomes, with an average marker density of 0.22 cM/marker. Linkage analysis allowed the identification of three different regions significantly associated with leaf rust resistance, with MG5323 contributing the resistant alleles. A major resistance gene was detected on the short arm of chromosome 1B, explaining a total phenotypic variation ranging from 41.37 to 49.51 %. Two additional minor resistance genes located on chromosome 7B explained a phenotypic variation ranging between 17.77 and 25.81 %. No obvious positional relationships were observed when the map position of the genes was compared with those of other previously identified wheat leaf rust resistance genes, suggesting that new resistance sources to leaf rust were identified in the tetraploid background. A significant positive epistatic effect was detected between quantitative trait loci (QTLs) for each trait, indicating that different QTLs contribute different degrees of resistance. Analysis of the leaf rust responses of the RILs demonstrated that only lines bearing resistant alleles at both loci showed effective leaf rust resistance, indicating that the genes identified behave as complementary genes.
Publisher version (URL)
Identifiersdoi: 10.1007/s11032-014-0186-0
issn: 1380-3743
Appears in Collections:(IAS) Artículos

Files in This Item:
File Description SizeFormat
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
Show full item record
Review this work

Google ScholarTM




WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.