English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/127596
Título

Discovering hidden biodiversity: the use of complementary monitoring of fish diet based on DNA barcoding in freshwater ecosystems

AutorJo, Hyunbin; Ventura, Marc ; Vidal, Nicolas; Gim, Jeong-Soo; Buchaca, Teresa ; Barmuta, L.A.; Jeppesen, Erik; Joo, Gea-Jae
Palabras claveDNA barcoding
Shallow lakes
Fish diet
Monitoring tool
Fecha de publicación2016
EditorJohn Wiley & Sons
CitaciónEcology and Evolution : doi: 10.1002/ece3.1825 (2016)
ResumenEcological monitoring contributes to the understanding of complex ecosystem functions. The diets of fish reflect the surrounding environment and habitats and may, therefore, act as useful integrating indicators of environmental status. It is, however, often difficult to visually identify items in gut contents to species level due to digestion of soft-bodied prey beyond visual recognition, but new tools rendering this possible are now becoming available. We used a molecular approach to determine the species identities of consumed diet items of an introduced generalist feeder, brown trout (Salmo trutta), in 10 Tasmanian lakes and compared the results with those obtained from visual quantification of stomach contents. We obtained 44 unique taxa (OTUs) belonging to five phyla, including seven classes, using the barcode of life approach from cytochrome oxidase I (COI). Compared with visual quantification, DNA analysis showed greater accuracy, yielding a 1.4-fold higher number of OTUs. Rarefaction curve analysis showed saturation of visually inspected taxa, while the curves from the DNA barcode did not saturate. The OTUs with the highest proportions of haplotypes were the families of terrestrial insects Formicidae, Chrysomelidae, and Torbidae and the freshwater Chironomidae. Haplotype occurrence per lake was negatively correlated with lake depth and transparency. Nearly all haplotypes were only found in one fish gut from a single lake. Our results indicate that DNA barcoding of fish diets is a useful and complementary method for discovering hidden biodiversity.
Descripción14 páginas, 4 figuras, 5 tablas
Versión del editorhttp://dx.doi.org/10.1002/ece3.1825
URIhttp://hdl.handle.net/10261/127596
DOI10.1002/ece3.1825
ISSN2045-7758
E-ISSN2045-7758
Aparece en las colecciones: (CEAB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Buchaca 2016.pdf2,58 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.