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Título

Unravelling the proteomic profile of rice meiocytes during early meiosis

AutorCollado-Romero, Melania ; Alós, Enriqueta ; Prieto, Pilar
Palabras claveMS/MS
Meiocytes proteome
SKP1 proteins
Cytoskeleton
Prophase I
Chromosome recognition
Chromosome pairing
Fecha de publicación24-jul-2014
EditorFrontiers Media
CitaciónFrontiers in Plant Science 5: 356 (2014)
ResumenTransfer of genetic traits from wild or related species into cultivated rice is nowadays an important aim in rice breeding. Breeders use genetic crosses to introduce desirable genes from exotic germplasms into cultivated rice varieties. However, in many hybrids there is only a low level of pairing (if existing) and recombination at early meiosis between cultivated rice and wild relative chromosomes. With the objective of getting deeper into the knowledge of the proteins involved in early meiosis, when chromosomes associate correctly in pairs and recombine, the proteome of isolated rice meiocytes has been characterized by nLC-MS/MS at every stage of early meiosis (prophase I). Up to 1316 different proteins have been identified in rice isolated meiocytes in early meiosis, being 422 exclusively identified in early prophase I (leptotene, zygotene, or pachytene). The classification of proteins in functional groups showed that 167 were related to chromatin structure and remodeling, nucleic acid binding, cell-cycle regulation, and cytoskeleton. Moreover, the putative roles of 16 proteins which have not been previously associated to meiosis or were not identified in rice before, are also discussed namely: seven proteins involved in chromosome structure and remodeling, five regulatory proteins [such as SKP1 (OSK), a putative CDK2 like effector], a protein with RNA recognition motifs, a neddylation-related protein, and two microtubule-related proteins. Revealing the proteins involved in early meiotic processes could provide a valuable tool kit to manipulate chromosome associations during meiosis in rice breeding programs. The data have been deposited to the ProteomeXchange with the PXD001058 identifier.
Versión del editorhttp://dx.doi.org/10.3389/fpls.2014.00356
URIhttp://hdl.handle.net/10261/127577
DOI10.3389/fpls.2014.00356
Identificadoresdoi: 10.3389/fpls.2014.00356
issn: 1664-462X
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