English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/127478
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Effects of Fe deficiency on the protein profile of Brassica napus phloem sap

AuthorsGutiérrez-Carbonell, Elaín; Lattanzio, Giuseppe ; Albacete, Alfonso ; Ríos Ruiz, Juan José ; Kehr, Julia; Abadía Bayona, Anunciación ; Grusak, Michael A.; Abadía Bayona, Javier ; López-Millán, Ana Flor
Issue DateNov-2015
PublisherJohn Wiley & Sons
CitationGutiérrez-Carbonell E, Lattanzio G, Albacete A, Ríos JJ, Kehr J, Abadía A, Grusak MA, Abadía J, López-Millán AF. Effects of Fe deficiency on the protein profile of Brassica napus phloem sap. Proteomics 15 (22): 3755–3920 (2015)
AbstractThe aim of this work was to study the effect of Fe deficiency on the protein profile of phloem sap exudates from Brassica napus using 2DE (IEF-SDS-PAGE). The experiment was repeated thrice and two technical replicates per treatment were done. Phloem sap purity was assessed by measuring sugar concentrations. Two hundred sixty-three spots were consistently detected and 15.6% (41) of them showed significant changes in relative abundance (22 decreasing and 19 increasing) as a result of Fe deficiency. Among them, 85% (35 spots), were unambiguously identified. Functional categories containing the largest number of protein species showing changes as a consequence of Fe deficiency were signaling and regulation (32%), and stress and redox homeostasis (17%). The Phloem sap showed a higher oxidative stress and significant changes in the hormonal profile as a result of Fe deficiency. Results indicate that Fe deficiency elicits major changes in signaling pathways involving Ca and hormones, which are generally associated with flowering and developmental processes, causes an alteration in ROS homeostasis processes, and induces decreases in the abundances of proteins involved in sieve element repair, suggesting that Fe-deficient plants may have an impaired capacity to heal sieve elements upon injury.
Description19 Pags.- 5 Tabls.- 4 Figs.
Publisher version (URL)http://dx.doi.org/10.1002/pmic.201400464
Appears in Collections:(EEAD) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.