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Electrochemical antibody-aptamer assay for VEGF cancer biomarker detection

AuthorsRavalli, Andrea; Marrazza, Giovanna; Rivas, Lourdes; Escosura-Muñiz, Alfredo de la ; Merkoçi, Arben
Issue Date2014
CitationSensors and Microsystems: 175-178 (2014)
SeriesLecture Notes in Electrical Engineering
AbstractIn this work, a simple and sensitive approach for VEGF detection using antibody-aptamer assay and gold screen-printed electrodes as transducers is presented. The assay was performed in a sandwich format. Anti-VEGF antibody was first chemically immobilized on the gold working electrode surface of screen-printed cell. After the incubation with the antigen, the sandwich assay was realized by incubation step with biotinylated anti-VEGF aptamer. The sensor was then incubated with streptavidin-alkaline phosphatase and with 1-naphthyl phosphate. Differential pulse voltammetry (DPV) measurements were performed to detect VEGF biomarker.
DescriptionProceedings of the 17th National Conference, Brescia (Italy), 5-7 February 2013.
Identifiersdoi: 10.1007/978-3-319-00684-0_33
isbn: 978-3-319-00683-3
Appears in Collections:(CIN2) Libros y partes de libros
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