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Electrochemical antibody-aptamer assay for VEGF cancer biomarker detection

AutorRavalli, Andrea; Marrazza, Giovanna; Rivas, Lourdes; Escosura-Muñiz, Alfredo de la ; Merkoçi, Arben
Fecha de publicación2014
EditorSpringer
CitaciónSensors and Microsystems: 175-178 (2014)
SerieLecture Notes in Electrical Engineering
268
ResumenIn this work, a simple and sensitive approach for VEGF detection using antibody-aptamer assay and gold screen-printed electrodes as transducers is presented. The assay was performed in a sandwich format. Anti-VEGF antibody was first chemically immobilized on the gold working electrode surface of screen-printed cell. After the incubation with the antigen, the sandwich assay was realized by incubation step with biotinylated anti-VEGF aptamer. The sensor was then incubated with streptavidin-alkaline phosphatase and with 1-naphthyl phosphate. Differential pulse voltammetry (DPV) measurements were performed to detect VEGF biomarker.
DescripciónProceedings of the 17th National Conference, Brescia (Italy), 5-7 February 2013.
URIhttp://hdl.handle.net/10261/126867
DOI10.1007/978-3-319-00684-0_33
Identificadoresdoi: 10.1007/978-3-319-00684-0_33
isbn: 978-3-319-00683-3
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