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Title

Electrocatalytic tuning of biosensing response through electrostatic or hydrophobic enzyme-graphene oxide interactions

AuthorsBaptista-Pires, Luis ; Pérez-López, Briza ; Mayorga-Martínez, Carmen C. ; Morales-Narváez, Eden ; Domingo, Neus ; Esplandiú, María J. ; Alzina, Francesc ; Sotomayor Torres, C. M. ; Merkoçi, Arben
KeywordsElectrocatalytic tuning
Oxidized graphene oxide
Hydrophobic interactions
Electrostatic interactions
Reduced graphene oxide
Issue Date2014
PublisherElsevier
CitationBiosensors and Bioelectronics 61: 655-662 (2014)
AbstractThe effect of graphene oxidative grades upon the conductivity and hydrophobicity and consequently the influence on an enzymatic biosensing response is presented. The electrochemical responses of reduced graphene oxide (rGO) have been compared with the responses obtained from the oxide form (oGO) and their performances have been accordingly discussed with various evidences obtained by optical techniques. We used tyrosinase enzyme as a proof of concept receptor with interest for phenolic compounds detection through its direct adsorption onto a screen-printed carbon electrode previously modified with oGO or rGO with a carbon-oxygen ratio of 1.07 or 1.53 respectively. Different levels of oGO directly affect the (bio)conjugation properties of the biosensor due to changes at enzyme/graphene oxide interface coming from the various electrostatic or hydrophobic interactions with biomolecules. The developed biosensor was capable of reaching a limit of detection of 0.01. nM catechol. This tuning capability of the biosensor response can be of interest for building several other biosensors, including immunosensors and DNA sensors for various applications. © 2014 Elsevier B.V.
URIhttp://hdl.handle.net/10261/126863
DOI10.1016/j.bios.2014.05.028
Identifiersdoi: 10.1016/j.bios.2014.05.028
issn: 0956-5663
e-issn: 1873-4235
Appears in Collections:(CIN2) Artículos
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