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Evaluation of semiconductor nanocrystals and supermagnetic nanoparticles for the development of new diagnostic and control methods of plant and human fungal pathogens

AutorRispail, Nicolas ; Rubiales, Diego ; Prats, Elena
Fecha de publicación12-feb-2014
CitaciónNanoscience and Nanotechnology International Conference (2014)
ResumenRecent developments in nanotechnology have demonstrated the usefulness of nanoparticles (NPs) for a wide range of application in biological systems [1,2,3]. All these applications make NPs promising tools for the development of novel strategies for the detection and control of plant and human diseases. However, before the design of such strategies, it is crucial to elucidate their interactions with target cells, cellular uptake and/or eventual excretion from cells, and the possible induction of toxic biological responses. Thus, we evaluated the interaction of two types of NPs, semiconductor nanocrystals (QDs) and superparamagnetic NPs (SiO2/MgO-magnetite) with fungal cells, as essential step to achieve the early detection of fungal pathogens and to address the feasibility of new NP-based smart delivery systems for its control. QDs were chosen because of their high sensitivity for detection in optical microscopy while the superparamagnetic NPs are biocompatible magnetic labels for magnetic detection. NPs were tested with Fusarium oxysporum, an important plant fungal pathogen responsible for economically devastating vascular wilts of most crops [4] and an opportunistic pathogen of immunecompromised patients [5]. No completely efficient control methods are available so far. Thus, an early diagnostic of the pathogen is crucial for its control. Our results indicated a differential trend in NP internalization and toxicity depending on the NPs. Imaging of the F. oxysporum-NP interaction by means of confocal microscopy indicated that both types of NP showed high affinity for the pathogen and were quickly attracted by the fungal cells (Fig. 1).However, while the QDs readily penetrated the fungal hyphae, most of the SiO2/MgO-magnetite NPs remained attached to the fungal cell wall surface (Figs. 1).Toxicological assessment by evaluation of fungal germination and growth, production and accumulation of ROS, and cell viability indicated no or mild toxicity of both types of NPs at biological concentration. Altogether, the internalization and toxicity studies showed that the QDs and SiO2/MgO-magnetite NPs might be applied for the rapid and sensitive detection of F. oxysporum. After adequate functionalization, they may also be useful for the control of this devastating pathogen. In both cases, in combination with a biomolecule able to target a desired formae specialis, the NPs could act as inner-(in the case of QDs) or -surface (in the case of magnetic NPs) fungal labels. To this purposes, antibodies directed against a highly specific pathogenicity-related protein of the fungus have been obtained and used to functionalize both types of NPs. The preliminary results on their functionalization and detection of the functionalized forms are also presented.
DescripciónRispail, Nicolas et al.
Trabajo presentado en la Nanoscience and Nanotechnology International Conference (NanoPT2014), celebrada en Oporto del 12 al 14 de febrero de 2014
URIhttp://hdl.handle.net/10261/126711
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