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Label-free electrochemical DNA sensor using "click"-functionalized PEDOT electrodes

AutorGalán, Teresa R.; Prieto-Simón, Beatriz; Alvira, Margarita; Eritja Casadellà, Ramón; Götz, Günther; Bäuerle, Peter; Samitier, Josep
Palabras claveAzido-EDOT
DNA biosensor
Click chemistry
Electrochemistry
Differential pulse voltammetry
Hepatitis C virus
Fecha de publicación5-dic-2015
EditorElsevier
CitaciónBiosensors and Bioelectronics
ResumenHere we describe a label-free electrochemical DNA sensor based on poly(3,4-ethylenedioxythiophene)-modified (PEDOT-modified) electrodes. An acetylene-terminated DNA probe, complementary to a specific "Hepatitis C" virus sequence, was immobilized onto azido-derivatized conducting PEDOT electrodes using "click" chemistry. DNA hybridization was then detected by differential pulse voltammetry, evaluating the changes in the electrochemical properties of the polymer produced by the recognition event. A limit of detection of 0.13. nM was achieved using this highly selective PEDOT-based genosensor, without the need for labeling techniques or microelectrode fabrication processes. These results are promising for the development of label-free and reagentless DNA hybridization sensors based on conducting polymeric substrates. Biosensors can be easily prepared using any DNA sequence containing an alkyne moiety. The data presented here reveal the potential of this DNA sensor for diagnostic applications in the screening of diseases, such as "Hepatitis C", and genetic mutations.
Versión del editorDOI: 10.1016/j.bios.2015.07.037
URIhttp://hdl.handle.net/10261/126695
DOI10.1016/j.bios.2015.07.037
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