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Electrostatic and hydrophobic interactions involved in CNT biofunctionalization with short ss-DNA

AuthorsCarot, M. Lucrecia; Esplandiú, María J. ; Giacomelli, Carla E.
Issue Date2010
PublisherAmerican Chemical Society
CitationJournal of Physical Chemistry C 114(10): 4459-4465 (2010)
AbstractThis work is aimed at studying the adsorption mechanism of short chain 20-mer pyrimidinic homoss-DNA (oligodeoxyribonucleotide, ODN: polyC20 and polyT20) onto CNT by reflectometry. To analyze the experimental data, the effective-medium theory using the Bruggemann approximation represents a suitable optical model to account for the surface properties (roughness, thickness, and optical constants) and the size of the adsorbate. Systematic information about the involved interactions is obtained by changing the physicochemical properties of the system. Hydrophobic and electrostatic interactions are evaluated by comparing the adsorption on hydrophobic CNT and on hydrophilic silica and by modulating the ionic strength with and without Mg2+. The ODN adsorption process on CNT is driven by hydrophobic interactions only when the electrostatic repulsion is suppressed. The adsorption mode results in ODN molecules in a side-on orientation with the bases (nonpolar region) toward the surface. This unfavorable orientation is partially reverse by adding Mg2+. On the other hand, the adsorption on silica is dominated by the strong repulsive electrostatic interaction that is screened at high ionic strength or mediated by Mg2+. The cation-mediated process induces the interaction of the phosphate backbone (polar region) with the surface, leaving the bases free for hybridization. Although the general adsorption behavior of the pyrimidine bases is the same, polyC20 presents higher affinity for the CNT surface due to its acid-base properties.
Identifiersdoi: 10.1021/jp9085359
issn: 1932-7447
e-issn: 1932-7455
Appears in Collections:(CIN2) Artículos
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