English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/126493
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


RNA/aTNA chimeras: RNAi effects and nucleases resistance of single and double stranded RNAs

AuthorsAlagia, Adele ; Eritja Casadellà, Ramón ; Terrazas, Montserrat
3'-overhang chemical modification
ApoB gene
Serum resistance
Single-stranded siRNA
Issue DateNov-2014
PublisherMultidisciplinary Digital Publishing Institute
AbstractThe RNA interference pathway (RNAi) is a specific and powerful biological process, triggered by small non-coding RNA molecules and involved in gene expression regulation. In this work, we explored the possibility of increasing the biological stability of these RNA molecules by replacing their natural ribose ring with an acyclic L-threoninol backbone. In particular, this modification has been incorporated at certain positions of the oligonucleotide strands and its effects on the biological properties of the siRNA have been evaluated. In vitro cellular RNAi assays have demonstrated that the L-threoninol backbone is well tolerated by the RNAi machinery in both double and single-stranded fashion, with activities significantly higher than those evinced by the unmodified RNAs and comparable to the well-known phosphorothioate modification. Additionally, this modification conferred extremely strong resistance to serum and 3′/5′-exonucleases. In view of these results, we applied this modification to the knockdown of a therapeutically relevant human gene such as apolipoprotein B ( ApoB). Further studies on the activation of the innate immune system showed that L-threoninol-modified RNAs are slightly less stimulatory than unmodified RNAs.
Publisher version (URL)DOI: 10.3390/molecules191117872
Appears in Collections:(IQAC) Artículos
Files in This Item:
File Description SizeFormat 
molecules-19-17872threoninol.pdfArtículo principal1,55 MBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.