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Image Acquisition – do’s, don’ts, and important things to remember

AutorClausen, Lotte Worsøe; Lombarte, Antoni
Fecha de publicación21-oct-2014
EditorInternational Council for the Exploration of the Sea
Citación5th International Otolith Symposium. Book Abstracts: 284-285 (2014)
ResumenOtolith shape analysis is a widely used technique for e.g. species- or stock identification and separation. There are several steps in shape analysis which each are equally important for the validity, ease and efficiency of the result from any analyses of otolith shape as they all potentially can bias the perception of the actual otolith shape. Here we demonstrate how the image acquisition process can introduce artefacts if not carefully considered prior to any analysis of otolith shape. The steps involved in acquiring standardised images for otolith shape analysis are demonstrated with particular focus on contour acquisition by light microscope of the whole otolith and sulcus acusticus (internal side) shapes of otoliths. Potential caveats in relation to the physical otolith will be identified (e.g. anatomical position, side asymmetries, crystallizations anomalies, broken tips, otolith rotation in relation to curvature) as well as important factors to consider in relation to the image acquisition set-up (e.g. light settings, background and medium) and approaches to dealing with this error will be outlined. Additionally a demonstration of methods for capture of internal otolith shape will be made along with guidance as to how to identify the optimal structures for analysis. The session will be a combination of presentations and live demonstration of image acquisition and image digital processing illustrating a series of issues to take into consideration when acquiring photos of otoliths. The aim is for participants to leave with an outline of what to include when setting up a procedure for otolith shape acquisition minimising the potential bias of image artefacts on the following otolith shape descriptor extractions
Descripción5th International Otolith Symposium (IOS2014), 20-24 October 2014, Mallorca, Balearic Islands Spain.-- 2 pages
Versión del editorhttp://www.ices.dk/news-and-events/symposia/otolith/Pages/Programme.aspx
Aparece en las colecciones: (ICM) Comunicaciones congresos
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