English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/126291
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

Haemophilus influenzae responds to glucocorticoids used in asthma therapy by modulation of biofilm formation and antibiotic resistance

AutorEarl, Chris S.; Garmendia, Juncal ; Ryan, Robert P.
Palabras claveAntibiotic resistance
Asthma
Biofilm
Haemophilus influenzae
Steroids
Fecha de publicación1-ago-2015
EditorEuropean Molecular Biology Organization
CitaciónEMBO Molecular Medicine 7(8): 1018-1033 (2015)
ResumenGlucocorticosteroids are used as a main treatment to reduce airway inflammation in people with asthma who suffer from neutrophilic airway inflammation, a condition frequently associated with Haemophilus influenzae colonization. Here we show that glucocorticosteroids have a direct influence on the behavior of H. influenzae that may account for associated difficulties with therapy. Using a mouse model of infection, we show that corticosteroid treatment promotes H. influenzae persistence. Transcriptomic analysis of bacteria either isolated from infected mouse airway or grown in laboratory medium identified a number of genes encoding regulatory factors whose expression responded to the presence of glucocorticosteroids. Importantly, a number of these corticosteroid-responsive genes also showed elevated expression in H. influenzae within sputum from asthma patients undergoing steroid treatment. Addition of corticosteroid to H. influenzae led to alteration in biofilm formation and enhanced resistance to azithromycin, and promoted azithromycin resistance in an animal model of respiratory infection. Taken together, these data strongly suggest that H. influenzae can respond directly to corticosteroid treatment in the airway potentially influencing biofilm formation, persistence and the efficacy of antibiotic treatment.
DescripciónEarl, Chris S. et al.
Versión del editorhttp://dx.doi.org/10.15252/emmm.201505088
URIhttp://hdl.handle.net/10261/126291
DOI10.15252/emmm.201505088
Identificadorese-issn: 1757-4684
issn: 1757-4676
Aparece en las colecciones: (IDAB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Haemophilus influenzae_Earl.pdf1,23 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.