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Título

Fast regulation of AP-1 activity through interaction of lamin A/C, ERK1/2, and c-Fos at the nuclear envelope

AutorGonzález, José M.; Navarro, Ana; Casar, Berta ; Crespo, Piero ; Andrés, Vicente
Palabras claveAP-1
A-type lamins
c-Fos
ERK1/2
Nuclear envelope
Fecha de publicación17-nov-2008
EditorRockefeller University Press
CitaciónJournal of Cell Biology 183(4): 653-66 (2008)
ResumenSequestration of c-Fos at the nuclear envelope (NE) through interaction with A-type lamins suppresses AP-1-dependent transcription. We show here that c-Fos accumulation within the extraction-resistant nuclear fraction (ERNF) and its interaction with lamin A are reduced and enhanced by gain-of and loss-of ERK1/2 activity, respectively. Moreover, hindering ERK1/2-dependent phosphorylation of c-Fos attenuates its release from the ERNF induced by serum and promotes its interaction with lamin A. Accordingly, serum stimulation rapidly releases preexisting c-Fos from the NE via ERK1/2-dependent phosphorylation, leading to a fast activation of AP-1 before de novo c-Fos synthesis. Moreover, lamin A-null cells exhibit increased AP-1 activity and reduced levels of c-Fos phosphorylation. We also find that active ERK1/2 interacts with lamin A and colocalizes with c-Fos and A-type lamins at the NE. Thus, NE-bound ERK1/2 functions as a molecular switch for rapid mitogen-dependent AP-1 activation through phosphorylation-induced release of preexisting c-Fos from its inhibitory interaction with lamin A/C.
DescripciónThis article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication.
Versión del editorhttp://dx.doi.org/10.1083/jcb.200805049
URIhttp://hdl.handle.net/10261/12626
DOI10.1083/jcb.200805049
ISBN0021-9525
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