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Title

Alteration by thioredoxin f over-expression of primary carbon metabolism and its response to elevated CO2 in tobacco (Nicotiana tabacum L.)

AuthorsAranjuelo, Iker ; Tcherkez, Guillaume; Jauregui, Iván; Gilard, Françoise; Ancín, María ; Fernández San Millán, Alicia ; Larraya, Luis; Veramendi, Jon ; Farrán, Inmaculada
KeywordsMetabolomics
Photosynthesis
Rubisco
Thioredoxin f
Tobacco
Issue DateOct-2015
PublisherElsevier
CitationEnvironmental and Experimental Botany 118: 40-48 (2015)
AbstractThioredoxins f (Trx f) are chloroplastic proteins that have been shown to be essential for the redox-based regulation of several steps of carbon assimilation, such as the Calvin-Benson-Bassham cycle. However, the effective impact of Trx f activity on photosynthetic performance and carbon primary metabolism, including under varying CO<inf>2</inf> mole fraction, is not well documented. In this study, we provide a physiological and metabolomic characterization of leaves in transplastomic Trx f over-expressing tobacco (Nicotiana tabacum L., cv. Petit Havana SR1) grown under either ambient or elevated CO<inf>2</inf> (400 or 800μmolmol<sup>-1</sup>). Trx f overexpression strongly increased starch synthesis under both ambient and elevated CO<inf>2</inf> but was not accompanied by a stimulation of net photosynthetic CO<inf>2</inf> fixation. Rather, Trx f-overexpressing plants had a lower photorespiration rate due to an increase in internal (mesophyll) conductance for CO<inf>2</inf> (with the consequent increase in CO<inf>2</inf> mole fraction at the carboxylation site, c<inf>c</inf>), and a higher decrease (compared with the wild-type) in total photosynthetic electron flux upon acclimation to elevated CO<inf>2</inf>. There were also changes in a number of metabolites, such as enrichment in sugar phosphates and free phosphate, and depletion in alanine, threonine and free sugars. Our results suggest that over-expressing Trx f has an influence on chloroplastic metabolism by simultaneously stimulating the carboxylation-to-oxygenation ratio and starch synthesis, with side effects on amino acid metabolism. The potential mechanisms involved are discussed.
Publisher version (URL)http://dx.doi.org/10.1016/j.envexpbot.2015.05.008
URIhttp://hdl.handle.net/10261/126235
DOI10.1016/j.envexpbot.2015.05.008
Identifiersissn: 0098-8472
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