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Título

Fluorescent fingerprints of endolithic phototrophic cyanobacteria living within halite rocks in the Atacama Desert

AutorRoldán, M.; Ascaso, Carmen ; Wierzchos, Jacek
Fecha de publicación7-mar-2014
EditorAmerican Society for Microbiology
CitaciónApplied and Environmental Microbiology 80(10): 2998-3006 (2014)
ResumenHalite deposits from the hyperarid zone of the Atacama Desert reveal the presence of endolithic microbial colonization dominated by cyanobacteria associated with heterotrophic bacteria and archaea. Using the λ-scan confocal laser scanning microscopy (CLSM) option, this study examines the autofluorescence emission spectra produced by single cyanobacterial cells found inside halite rocks and by their photosynthetic pigments. Photosynthetic pigments could be identified according to the shapes of the emission spectra and wavelengths of fluorescence peaks. According to their fluorescence fingerprints, three groups of cyanobacterial cells were identified within this natural extreme microhabitat: (i) cells producing a single fluorescence peak corresponding to the emission range of phycobiliproteins and chlorophyll a, (ii) cells producing two fluorescence peaks within the red and green signal ranges, and (iii) cells emitting only low-intensity fluorescence within the nonspecific green fluorescence signal range. Photosynthetic pigment fingerprints emerged as indicators of the preservation state or viability of the cells. These observations were supported by a cell plasma membrane integrity test based on Sytox Green DNA staining and by transmission electron microscopy ultrastructural observations of cyanobacterial cells. © 2014, American Society for Microbiology.
URIhttp://hdl.handle.net/10261/126133
DOI10.1128/AEM.03428-13
Identificadoresdoi: 10.1128/AEM.03428-13
issn: 1098-5336
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