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Selection of a nucleopolyhedrovirus isolate from Helicoverpa armigera as the basis for a biological insecticide

AuthorsArrizubieta, Maite ; Williams, Trevor G.; Caballero, Primitivo ; Simón, Oihane
KeywordsHelicoverpa armigera
REN analysis
Physical map
Insecticidal properties
Ingredient active production
Issue Date13-Oct-2013
PublisherJohn Wiley & Sons
CitationPest Management Science 70(6): 967- 976 (2014)
AbstractBACKGROUND: The cotton bollworm, Helicoverpa armigera, is an insect that causes damage in a wide range of crops in Spain. Seven isolates of H. armigera single nucleopolyhedrovirus (HearSNPV) from the Iberian Peninsula were subjected to molecular and biological characterization and compared with a Chinese genotype (HearSNPV-G4). RESULTS: The estimated sizes of the Iberian genomes varied between 116.2 and 132.4 kb, compared to 131.4 kb of the HearSNPV-G4 reference genome. Phylogenetic analysis based on the lef-8, lef-9 and polh genes revealed that the Iberian strains were more closely related to one another than to other HearSNPV isolates. Occlusion body (OB) concentration-mortality responses (LC50 values) did not differ significantly among Iberian isolates when tested against a Helicoverpa armigera colony from Oxford (UK). Despite being the fastest killing isolate, HearSNPV-SP1 was as productive as isolates with lower virulence, with an average yield of 3.1¿×¿109 OBs larva-1 . OBs of HearSNPV-SP1 and HearSNPV-G4 were similarly pathogenic against a recently established colony from southern Spain, although HearSNPV-SP1 was faster killing than HearSNPV-G4 against a range of instars. CONCLUSION: The insecticidal properties of HearSNPV-SP1 mean that this strain is likely to prove useful as the basis for a biological insecticide for control of Helicoverpa armigera in Spain.
Publisher version (URL)http://dx.doi.org/10.1002/ps.3637
Identifiersdoi: 10.1002/ps.3637
issn: 1526-498X
Appears in Collections:(IDAB) Artículos
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