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dc.contributor.author | Bahaji, Abdellatif | - |
dc.contributor.author | Baroja-Fernández, Edurne | - |
dc.contributor.author | Sánchez-López, Ángela María | - |
dc.contributor.author | Muñoz Pérez, Francisco José | - |
dc.contributor.author | Li, Jun | - |
dc.contributor.author | Almagro, Goizeder | - |
dc.contributor.author | Montero, Manuel | - |
dc.contributor.author | Pozueta Romero, Javier | - |
dc.date.accessioned | 2015-11-26T08:32:01Z | - |
dc.date.available | 2015-11-26T08:32:01Z | - |
dc.date.issued | 2014-08-18 | - |
dc.identifier | doi: 10.1371/journal.pone.0104997 | - |
dc.identifier | issn: 1932-6203 | - |
dc.identifier.citation | PLoS ONE 9(8): e104997 (2014) | - |
dc.identifier.uri | http://hdl.handle.net/10261/125953 | - |
dc.description.abstract | In leaves, it is widely assumed that starch is the end-product of a metabolic pathway exclusively taking place in the chloroplast that (a) involves plastidic phosphoglucomutase (pPGM), ADPglucose (ADPG) pyrophosphorylase (AGP) and starch synthase (SS), and (b) is linked to the Calvin-Benson cycle by means of the plastidic phosphoglucose isomerase (pPGI). This view also implies that AGP is the sole enzyme producing the starch precursor molecule, ADPG. However, mounting evidence has been compiled pointing to the occurrence of important sources, other than the pPGI-pPGM-AGP pathway, of ADPG. To further explore this possibility, in this work two independent laboratories have carried out HPLC-MS/MS analyses of ADPG content in leaves of the near-starchless pgm and aps1 mutants impaired in pPGM and AGP, respectively, and in leaves of double aps1/pgm mutants grown under two different culture conditions. We also measured the ADPG content in wild type (WT) and aps1 leaves expressing in the plastid two different ADPG cleaving enzymes, and in aps1 leaves expressing in the plastid GlgC, a bacterial AGP. Furthermore, we measured the ADPG content in ss3/ss4/aps1 mutants impaired in starch granule initiation and chloroplastic ADPG synthesis. We found that, irrespective of their starch contents, pgm and aps1 leaves, WT and aps1 leaves expressing in the plastid ADPG cleaving enzymes, and aps1 leaves expressing in the plastid GlgC accumulate WT ADPG content. In clear contrast, ss3/ss4/aps1 leaves accumulated ca. 300 fold-more ADPG than WT leaves. The overall data showed that, in Arabidopsis leaves, (a) there are important ADPG biosynthetic pathways, other than the pPGI-pPGM-AGP pathway, (b) pPGM and AGP are not major determinants of intracellular ADPG content, and (c) the contribution of the chloroplastic ADPG pool to the total ADPG pool is low. | - |
dc.description.sponsorship | This research was partially supported by the grants [BIO2010-18239] from the Comisión Interministerial de Ciencia y Tecnología and Fondo Europeo de Desarrollo Regional (Spain) and [IIM010491.RI1] from the Government of Navarra, and by Iden Biotechnology. This research was also supported by Scientific Research on Innovative Areas [22114507] and Grants-in-Aid for Scientific Research (B) [22380186] from the Ministry of Education, Culture, Sports, Science and Technology, Japan. | - |
dc.publisher | Public Library of Science | - |
dc.relation.isversionof | Publisher's version | - |
dc.rights | openAccess | - |
dc.title | HPLC-MS/MS Analyses Show That the Near-Starchless aps1 and pgm Leaves Accumulate Wild Type Levels of ADPglucose: Further Evidence for the Occurrence of Important ADPglucose Biosynthetic Pathway(s) Alternative to the pPGI-pPGM-AGP Pathway | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1371/journal.pone.0104997 | - |
dc.relation.publisherversion | http://dx.doi.org/10.1371/journal.pone.0104997 | - |
dc.date.updated | 2015-11-26T08:32:04Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.rights.license | http://creativecommons.org/licenses/by/4.0/ | - |
dc.contributor.funder | Comisión Interministerial de Ciencia y Tecnología, CICYT (España) | - |
dc.contributor.funder | Nafarroako Gobernua | - |
dc.contributor.funder | Iden Biotechnology | - |
dc.contributor.funder | Ministry of Education, Culture, Sports, Science and Technology (Japan) | - |
dc.contributor.funder | European Commission | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100007273 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100001700 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.pmid | 25133777 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
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HPLC MSMS Analyses Show_Bahaji.pdf | 1,1 MB | Adobe PDF | Visualizar/Abrir |
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