Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/125897
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Calero, Macarena | - |
dc.contributor.author | Chiappi, Michele | - |
dc.contributor.author | Lázaro-Nogal, Ana | - |
dc.contributor.author | Rodríguez, María Josefa | - |
dc.contributor.author | Chichón, Javier | - |
dc.contributor.author | Crosbie-Staunton, Kieran | - |
dc.contributor.author | Prina-Mello, Adriele | - |
dc.contributor.author | Volkov, Yuri | - |
dc.contributor.author | Villanueva, Ángeles | - |
dc.contributor.author | Carrascosa, José L. | - |
dc.date.accessioned | 2015-11-25T17:56:16Z | - |
dc.date.available | 2015-11-25T17:56:16Z | - |
dc.date.issued | 2015-02-26 | - |
dc.identifier.citation | Journal of Nanobiotechnology 13(1): 16 (2015) | - |
dc.identifier.issn | 1477-3155 | - |
dc.identifier.uri | http://hdl.handle.net/10261/125897 | - |
dc.description.abstract | [Background] Different superparamagnetic iron oxide nanoparticles have been tested for their potential use in cancer treatment, as they enter into cells with high effectiveness, do not induce cytotoxicity, and are retained for relatively long periods of time inside the cells. We have analyzed the interaction, internalization and biocompatibility of dimercaptosuccinic acid-coated superparamagnetic iron oxide nanoparticles with an average diameter of 15 nm and negative surface charge in MCF-7 breast cancer cells. | - |
dc.description.abstract | [Results] Cells were incubated with dimercaptosuccinic acid-coated superparamagnetic iron oxide nanoparticles for different time intervals, ranging from 0.5 to 72 h. These nanoparticles showed efficient internalization and relatively slow clearance. Time-dependent uptake studies demonstrated the maximum accumulation of dimercaptosuccinic acid-coated superparamagnetic iron oxide nanoparticles after 24 h of incubation, and afterwards they were slowly removed from cells. Superparamagnetic iron oxide nanoparticles were internalized by energy dependent endocytosis and localized in endosomes. Transmission electron microscopy studies showed macropinocytosis uptake and clathrin-mediated internalization depending on the nanoparticles aggregate size. MCF-7 cells accumulated these nanoparticles without any significant effect on cell morphology, cytoskeleton organization, cell cycle distribution, reactive oxygen species generation and cell viability, showing a similar behavior to untreated control cells. | - |
dc.description.abstract | [Conclusions] All these findings indicate that dimercaptosuccinic acid-coated superparamagnetic iron oxide nanoparticles have excellent properties in terms of efficiency and biocompatibility for application to target breast cancer cells. | - |
dc.description.sponsorship | The research leading to these results have received partial funding from the European Seventh Framework Programme (FP7/2007-2013) under the project MULTIFUN grant agreement no. 262943, and the project Nanofrontmag-CM (S2013/MIT-2850) from the Comunidad de Madrid. Additional grants were obtained from BFU 2011–29038 and CTQ2013-48767-C3-3-R from the Ministerio de Economia y Competitividad and S2009/Mat 1507 from the Comunidad de Madrid (to JLC), from EU FP7 project NAMDIATREAM (ref 246479) and from “la Caixa” / CNB International PhD Programme Fellowships. | - |
dc.publisher | BioMed Central | - |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/262943 | - |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/246479 | - |
dc.relation.isversionof | Publisher's version | - |
dc.rights | openAccess | - |
dc.subject | MCF-7 cells | - |
dc.subject | Superparamagnetic iron oxide nanoparticles | - |
dc.subject | Intracellular trafficking | - |
dc.subject | Transmission electron microscopy | - |
dc.subject | Cellular uptake | - |
dc.subject | Endocytosis | - |
dc.subject | Cytotoxicity | - |
dc.title | Characterization of interaction of magnetic nanoparticles with breast cancer cells | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1186/s12951-015-0073-9 | - |
dc.relation.publisherversion | http://dx.doi.org/10.1186/s12951-015-0073-9 | - |
dc.date.updated | 2015-11-25T17:56:16Z | - |
dc.language.rfc3066 | en | - |
dc.rights.holder | Calero et al.; licensee BioMed Central. | - |
dc.rights.license | http://creativecommons.org/licenses/by/4.0 | - |
dc.contributor.funder | European Commission | - |
dc.contributor.funder | Comunidad de Madrid | - |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | - |
dc.contributor.funder | CSIC - Centro Nacional de Biotecnología (CNB) | - |
dc.contributor.funder | La Caixa | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/100012818 | es_ES |
dc.identifier.pmid | 25880445 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.openairetype | artículo | - |
Aparece en las colecciones: | (CNB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
12951_2015_Article_73.pdf | 2,46 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
41
checked on 24-abr-2024
SCOPUSTM
Citations
97
checked on 19-abr-2024
WEB OF SCIENCETM
Citations
100
checked on 28-feb-2024
Page view(s)
303
checked on 23-abr-2024
Download(s)
385
checked on 23-abr-2024