English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/125850
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Revealing microbial recognition by specific antibodies

AuthorsSimón-Soro, Áurea; D’Auria, Giussepe; Collado, María Carmen ; Džunková, Mária; Culshaw, Shauna; Mira, Alex
KeywordsImmunoglobulin
Flow cytometry
Pyrosequencing
16S rRNA
Opsonization
Human microbiome
Issue Date2-Jul-2015
PublisherBioMed Central
CitationBMC Microbiology 15(1): 132 (2015)
Abstract[Background] Recognition of microorganisms by antibodies is a vital component of the human immune response. However, there is currently very limited understanding of immune recognition of 50 % of the human microbiome which is made up of as yet un-culturable bacteria. We have combined the use of flow cytometry and pyrosequencing to describe the microbial composition of human samples, and its interaction with the immune system.
[Results] We show the power of the technique in human faecal, saliva, oral biofilm and breast milk samples, labeled with fluorescent anti-IgG or anti-IgA antibodies. Using Fluorescence-Activated Cell Sorting (FACS), bacterial cells were separated depending on whether they are coated with IgA or IgG antibodies. Each bacterial population was PCR-amplified and pyrosequenced, characterizing the microorganisms which evade the immune system and those which were recognized by each immunoglobulin.
[Conclusions] The application of the technique to healthy and diseased individuals may unravel the contribution of the immune response to microbial infections and polymicrobial diseases.
Publisher version (URL)http://dx.doi.org/10.1186/s12866-015-0456-y
URIhttp://hdl.handle.net/10261/125850
DOI10.1186/s12866-015-0456-y
ISSN1471-2180
Appears in Collections:(IATA) Artículos
Files in This Item:
File Description SizeFormat 
12866_2015_Article_456.pdf1,46 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.