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Revealing microbial recognition by specific antibodies

AuthorsSimón-Soro, Áurea; D’Auria, Giussepe; Collado, María Carmen ; Džunková, Mária; Culshaw, Shauna; Mira, Alex
Flow cytometry
16S rRNA
Human microbiome
Issue Date2-Jul-2015
PublisherBioMed Central
CitationBMC Microbiology 15(1): 132 (2015)
Abstract[Background] Recognition of microorganisms by antibodies is a vital component of the human immune response. However, there is currently very limited understanding of immune recognition of 50 % of the human microbiome which is made up of as yet un-culturable bacteria. We have combined the use of flow cytometry and pyrosequencing to describe the microbial composition of human samples, and its interaction with the immune system.
[Results] We show the power of the technique in human faecal, saliva, oral biofilm and breast milk samples, labeled with fluorescent anti-IgG or anti-IgA antibodies. Using Fluorescence-Activated Cell Sorting (FACS), bacterial cells were separated depending on whether they are coated with IgA or IgG antibodies. Each bacterial population was PCR-amplified and pyrosequenced, characterizing the microorganisms which evade the immune system and those which were recognized by each immunoglobulin.
[Conclusions] The application of the technique to healthy and diseased individuals may unravel the contribution of the immune response to microbial infections and polymicrobial diseases.
Publisher version (URL)http://dx.doi.org/10.1186/s12866-015-0456-y
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