English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/125811
Título

Transient transformation of Podosphaera xanthii by electroporation of conidia

AutorVela-Corcía, David; Romero, Diego; Torés Montosa, Juan Antonio; Vicente, Antonio de; Pérez-García, Alejandro
Palabras claveElectrotransformation
Hygromycin B resistance
MBC resistance
Powdery mildew fungi
β-tubulin
Fecha de publicación6-feb-2015
EditorBioMed Central
CitaciónBMC Microbiology 15(1): 20 (2015)
Resumen[Background] Powdery mildew diseases are a major phytosanitary issue causing important yield and economic losses in agronomic, horticultural and ornamental crops. Powdery mildew fungi are obligate biotrophic parasites unable to grow on culture media, a fact that has significantly limited their genetic manipulation. In this work, we report a protocol based on the electroporation of fungal conidia, for the transient transformation of Podosphaera fusca (synonym Podosphaera xanthii), the main causal agent of cucurbit powdery mildew.
[Results] To introduce DNA into P. xanthii conidia, we applied two square-wave pulses of 1.7 kV for 1 ms with an interval of 5 s. We tested these conditions with several plasmids bearing as selective markers hygromycin B resistance (hph), carbendazim resistance (TUB2) or GFP (gfp) under control of endogenous regulatory elements from Aspergillus nidulans, Neurospora crassa or P. xanthii to drive their expression. An in planta selection procedure using the MBC fungicide carbendazim permitted the propagation of transformants onto zucchini cotyledons and avoided the phytotoxicity associated with hygromycin B.
[Conclusion] This is the first report on the transformation of P. xanthii and the transformation of powdery mildew fungi using electroporation. Although the transformants are transient, this represents a feasible method for the genetic manipulation of this important group of plant pathogens.
Versión del editorhttp://dx.doi.org/10.1186/s12866-014-0338-8
URIhttp://hdl.handle.net/10261/125811
DOI10.1186/s12866-014-0338-8
ISSN1471-2180
Aparece en las colecciones: (IHSM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
12866_2014_Article_338.pdf1,8 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.