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Exploring glycosignatures of pathogenic bacteria: the sweet side of biological recognition

AutorKalograiaki, Ioanna; Euba, Begoña ; Proverbio, Davide; Aastrup, Teodor; Garmendia, Juncal ; Solís, D.
Fecha de publicación28-nov-2014
Citación65th Congress of the Hellenic Society of Biochemistry and Molecular Biology (2014)
ResumenGlycan chains are gaining importance as recognition signals in numerous processes related to health and disease. In particular, specific glycosylation patterns are frequently found to be associated with pathogenesis, including pathogen-host interactions. Bacterial surfaces are coated with distinct signature carbohydrate structures, most prominently capsular polysaccharides and lipopolysaccharides (LPS), which are targeted by host receptors for triggering defence responses or are exploited by the pathogen as mechanism of attachment to the host-cell surface. We have established a proof-of-concept for the use of designer¿s microarrays in the exploration of glycosylation motifs of the bacterial surface. Non-typable Haemophilus influenzae (NTHi), an opportunistic pathogen frequently isolated from respiratory samples from chronic obstructive pulmonary disease patients, was selected as case-study. A library of mutant strains of the clinical isolate 375 lacking specific glycosyltransferases involved in the synthesis of the lipooligosaccharide (LOS) was generated. Isolated LOSs were quantified, characterised by polyacrylamide gel electrophoresis, and immobilised in the microarrays as probes, alongside the corresponding whole bacteria strains. As pattern-reading tool, a panel of plant lectins with well-defined oligosaccharide-binding specificities was used. A fingerprint-like binding profile for each NTHi strain was observed, and competition assays with lectin-specific haptens confirmed carbohydrate-dependent recognition. For selected bacteria-lectin pairs, a real-time kinetics study was performed with the novel biosensor platform Quartz Crystal Microgravimetry (QCM). Both microarray and QCM techniques allowed the evaluation of glycans¿ accessibility on the bacterial surface, a factor of significant impact on their biomarker potential and their recognition by endogenous lectins of the innate immune system. Typification of bacterial glycosignatures by this newly described approach may be of particular relevance when characterising clinical isolates or mutant libraries in order to establish virulence correlations.
DescripciónPóster presentado en el 65th Congress of the Hellenic Society of Biochemistry and Molecular Biology celebrado en Tesalónica (Grecia) los días del 28-30 de noviembre de 2014
URIhttp://hdl.handle.net/10261/125755
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