English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/125667
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Long-term effect of omega-3 fatty acid supplementation in a model of age-related hearing loss

AuthorsMartínez-Vega, Raquel ; Partearroyo, Teresa; Varela-Moreiras, Gregorio; Pajares, María A. ; Varela-Nieto, Isabel
KeywordsDietary supplementation
Oxidative stress
Issue Date2014
CitationIEB 2014
AbstractOmega-3 polyunsaturated fatty acids (PUFAs) are dietary nutrients well-known for their beneficial effects on cognitive development and maintenance, inflammation, oxidative stress and insulin sensitivity, among others. Insufficient levels of docosahexaenoic acid are associated to age-related neurological and vascular disorders, and with human age-related hearing loss (ARHL). There is an inverse relationship between high plasma levels of PUFAs and ARHL, whereas high levels of plasma homocysteine (tHcy) are associated with hearing loss. Here we used C57BL/6J mice and long-term Omega-3 supplementation to evaluate the impact on hearing, Hcy levels, oxidative stress and inflammation. Two-month old mice were fed either control or omega-3 supplemented diets for 10 months. Hearing capacity was assessed monthly by ABR and DPOAE threshold analyses. Blood samples were taken to measure tHcy and folate concentrations by HPLC. Cochlear morphology was evaluated with cresyl violet and immunohistochemistry. Biomarkers of inflammation and oxidative stress were analyzed by Western blotting and RT-qPCR. The control group showed significantly higher ABR hearing thresholds (25 dB SPL in average) and lower 2f1-f2 amplitude (nV) in mid-high frequencies, when compared to the Omega-3 group. No evident histological differences were found. Higher tHcy levels (p=0.13), together with decreased serum folate concentrations (p<0.05), were detected in control vs. Omega-3 supplemented mice. The results obtained suggest that omega-3 supplementation may have a long-term protective role on ARHL.
DescriptionPóster presentado al 51st Inner Ear Biology Workshop celebrado en Sheffield (UK) del 30 de agosto al 2 de septiembre de 2014.
Appears in Collections:(IIBM) Comunicaciones congresos
Files in This Item:
File Description SizeFormat 
Longtermloss.pdf1,76 MBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.