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Título

The composition of West Nile virus lipid envelope unveils a role of sphingolipid metabolism in flavivirus biogenesis

AutorMartín-Acebes, Miguel Ángel; Merino-Ramos, Teresa; Blázquez, Ana Belén; Casas, Josefina; Escribano-Romero, Estela; Sobrino Castelló, Francisco; Saiz, Juan-Carlos
Fecha de publicaciónoct-2014
EditorAmerican Society for Microbiology
CitaciónJournal of Virology 88: 12041- 12054 (2014)
Resumen© 2014, American Society for Microbiology. West Nile virus (WNV) is an emerging zoonotic mosquito-borne flavivirus responsible for outbreaks of febrile illness and meningoencephalitis. The replication of WNV takes place on virus-modified membranes from the endoplasmic reticulum of the host cell, and virions acquire their envelope by budding into this organelle. Consistent with this view, the cellular biology of this pathogen is intimately linked to modifications of the intracellular membranes, and the requirement for specific lipids, such as cholesterol and fatty acids, has been documented. In this study, we evaluated the impact of WNV infection on two important components of cellular membranes, glycerophospholipids and sphingolipids, by mass spectrometry of infected cells. A significant increase in the content of several glycerophospholipids (phosphatidylcholine, plasmalogens, and lysophospholipids) and sphingolipids (ceramide, dihydroceramide, and sphingomyelin) was noticed in WNV-infected cells, suggesting that these lipids have functional roles during WNV infection. Furthermore, the analysis of the lipid envelope of WNV virions and recombinant virus-like particles revealed that their envelopes had a unique composition. The envelopes were enriched in sphingolipids (sphingomyelin) and showed reduced levels of phosphatidylcholine, similar to sphingolipid-enriched lipid microdomains. Inhibition of neutral sphingomyelinase (which catalyzes the hydrolysis of sphingomyelin into ceramide) by either pharmacological approaches or small interfering RNA-mediated silencing reduced the release of flavivirus virions as well as virus-like particles, suggesting a role of sphingomyelin-to-ceramide conversion in flavivirus budding and confirming the importance of sphingolipids in the biogenesis of WNV.
URIhttp://hdl.handle.net/10261/125645
DOI10.1128/JVI.02061-14
Identificadoresdoi: 10.1128/JVI.02061-14
issn: 1098-5514
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