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Thyroid hormone action in cerebrocortical cells involves the transition from fetal to adult pattern of gene expression

AutorGil-Ibáñez, Pilar ; Bernal, Juan ; Morte, Beatriz
Fecha de publicación2014
CitaciónETA 2014
ResumenThyroid hormone (T3) is important during development of the mammalian brain acting through regulation of gene expression. T3 is involved in neuronal and glial cell differentiation and migration, axonal myelination, and synaptogenesis. To get further insight on the genes and pathways regulated by T3 in the developing brain at the cellular level, we used mouse cerebrocortical cells in primary culture. This culture maintains, to some extent, the original phenotypic cell diversity and therefore, reflects the action of T3 on the cortex in vivo. For example, 10% of the neurons are calbindin positive and T3 reduced calbindin expression without changing neuronal number. To identify thyroid hormone dependent genes we performed RNA-seq assays (Illumina). We have identified 619 genes that are upregulated by T3 as well as 526 genes that are downregulated by T3 (FRD<0.05). The technical and biological procedures were validated measuring the expression of 32 differentially express genes by RT-PCR. The Gene Ontology analysis revealed T3 upregulated genes involved in signal transduction such as synaptic transmission and ion transport and T3 downregulated genes involved in cellular proliferation. The upregulated genes contain a high proportion of genes enriched in the adult cortex. Conversely, the downregulated genes contain a high proportion of genes enriched in the embryonic brain. In this study we have identified many genes regulated at the cellular level by T3 in cerebrocortical primary cells. The results underscore the importance of T3 in the regulation of gene expression in stage-specific processes for a proper development and function of the brain.
DescripciónResumen del póster presentado al 38th Annual Meeting of the European Thyroid Association celebrado en Santiago de Compostela (España) del 6 al 10 de septiembre de 2014.-- et al.
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