Chemoenzymatic Synthesis and Antimicrobial and Haemolytic Activities of Amphiphilic Bis(phenylacetylarginine) Derivatives

Abstract

Novel bis(Nα-phenylacetyl-L-arginine)-α,w-alkanediamide dihydrochloride (bis(PhAcArg)) derivatives with antimicrobial activity were designed and synthesised by a chemoenzymatic strategy. The new structures consist of two Nα-phenylacetyl-L-arginine moieties connected by an alkanediamine spacer chain of 6, 8, 10, 12, and 14 methylene units through amide bonds. The key step in the chemoenzymatic strategy is the double aminolysis of the Nα-phenylacetyl-L-arginine methyl ester by the corresponding α,w-alkanediamine catalyzed by papain in ethanolic media. The compounds synthesised were tested as antimicrobials against 15 bacterial and 8 fungal species. The antimicrobial activity and selectivity depend strongly on the spacer chain length. The bis(PhAcArg) derivative with the spacer chain of 12 methylene groups gave the lowest MIC values against Gram-positive bacteria, whereas that with 14 methylene units was the best against Gram-negative bacteria.

Interestingly, these novel compounds showed enhanced antibacterial activity relative to the lead compound, bis(Nα-caproyl-L-arginine)-1,3-propanediamide dihydrochloride (C3(CA)2), and moderate antifungal activity. Moreover, tests of haemolytic activity toward human erythrocytes revealed that haemolysis increases with spacer chain length. Importantly, the compounds were classified as not irritating to eyes, with the exception of the compound with the spacer chain of 14 methylene groups, which was a slight eye irritant.