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Variable-Temperature Size Exclusion Chromatography for the Study of the Structural Changes in G-Quadruplex

AuthorsBenabou, Sanae; Gargallo, Raimundo; Eritja Casadellà, Ramón
Issue Date2013
PublisherHindawi Publishing Corporation
CitationISRN Biochemistry
AbstractThe conformational equilibria of a guanine-rich sequence found at the promoter region of the human c-kit oncogene are studied by means of circular dichroism spectroscopy (CD) and variable-temperature size exclusion chromatography (SEC). It is shown that the wild sequence ckit21 exists as a mixture of monomeric and multimeric G-quadruplexes. Appropriate mutation of several bases in the wild sequence produces the shift from parallel to antiparallel G-quadruplex, as well as the disappearance of multimeric species. The shift from the antiparallel to the parallel conformation induced by temperature is reflected in both CD and SEC profiles.
Publisher version (URL)http://dx.doi.org/10.1155/2013/631875
Appears in Collections:(IQAC) Artículos
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