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Title: | Variable-Temperature Size Exclusion Chromatography for the Study of the Structural Changes in G-Quadruplex |
Authors: | Benabou, Sanae; Gargallo, Raimundo; Eritja Casadellà, Ramón ![]() |
Keywords: | G-quadruplex chromatography |
Issue Date: | 2013 |
Publisher: | Hindawi Publishing Corporation |
Citation: | ISRN Biochemistry |
Abstract: | The conformational equilibria of a guanine-rich sequence found at the promoter region of the human c-kit oncogene are studied by means of circular dichroism spectroscopy (CD) and variable-temperature size exclusion chromatography (SEC). It is shown that the wild sequence ckit21 exists as a mixture of monomeric and multimeric G-quadruplexes. Appropriate mutation of several bases in the wild sequence produces the shift from parallel to antiparallel G-quadruplex, as well as the disappearance of multimeric species. The shift from the antiparallel to the parallel conformation induced by temperature is reflected in both CD and SEC profiles. |
Publisher version (URL): | http://dx.doi.org/10.1155/2013/631875 |
URI: | http://hdl.handle.net/10261/125376 |
DOI: | http://dx.doi.org/10.1155/2013/631875 |
Appears in Collections: | (IQAC) Artículos |
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ISRN Biochemistry 2013 631875.pdf | Artículo principal | 2,89 MB | Adobe PDF | ![]() View/Open |
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