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dc.contributor.authorVengut-Climent, Empares_ES
dc.contributor.authorTerrazas, Montserrates_ES
dc.contributor.authorLucas, Ricardoes_ES
dc.contributor.authorArévalo-Ruiz, Matildees_ES
dc.contributor.authorEritja Casadellà, Ramónes_ES
dc.contributor.authorMorales, Juan Carloses_ES
dc.date.accessioned2015-11-20T08:06:41Z-
dc.date.available2015-11-20T08:06:41Z-
dc.date.issued2013-07-
dc.identifier.citationBioorganic and Medicinal Chemistry Letterses_ES
dc.identifier.urihttp://hdl.handle.net/10261/125374-
dc.description.abstractOligoribonucleotide conjugates carrying apolar carbohydrates at the 5′-end and the corresponding siRNA duplexes have been prepared using phosphoramidite chemistry. All the carbohydrate-siRNA derivatives were compatible with RNA interference machinery if transfected with oligofectamine. In the absence of a transfection agent, some of them exerted certain reduction of gene expression. Double-tailed permethylated glucose conjugated to siRNA through a long spacer inhibited gene expression up to 26% compared to the scrambled duplex. Such modifications contribute positively to the stability of oligoribonucleotides against 5′-exonuclease degradation.es_ES
dc.description.sponsorshipThis study was supported by the Spanish Ministry of Economy and Competitiveness (grants CTQ2009-13705, CTQ2010-20541), Generalitat de Catalunya (2009/SGR/208). RL thanks CSIC for a JAE contract and EVC thanks Ministry of Education, Culture and Sports for a FPU grant. MT acknowledges a Juan de la Cierva contract (MICINN, Spain) for financial support.es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relation.isversionofPostprintes_ES
dc.rightsopenAccesses_ES
dc.subjectCarbohydratees_ES
dc.subjectRNAes_ES
dc.subjectLipophilic vectores_ES
dc.subjectsiRNAes_ES
dc.subjectModified siRNAes_ES
dc.subjectPhosphoramiditees_ES
dc.titleSynthesis, RNAi activity and nuclease-resistant properties of apolar carbohydrates siRNA conjugateses_ES
dc.typeartículoes_ES
dc.identifier.doi10.1016/j.bmcl.2013.05.065-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionDOI: 10.1016/j.bmcl.2013.05.065es_ES
dc.rights.licensehttp://www.sherpa.ac.uk/romeo/issn/0960-894X/es_ES
dc.relation.csices_ES
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